| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ABL, JTK7, p150, c-ABL, |
| Entrez GeneID | 25; |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
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以下是3篇关于c-Abl(Phospho-Tyr412)抗体的参考文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*"Activation of the c-Abl tyrosine kinase requires phosphorylation of Y412 in the kinase domain"*
**作者**：Dorey K, et al.
**摘要**：该研究通过结构分析和突变实验，证明c-Abl激酶活性依赖于Tyr412位点的磷酸化。使用Phospho-Tyr412特异性抗体验证了该位点的自磷酸化对激酶构象及底物结合的关键作用，并揭示了其在白血病相关突变中的调控异常。
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2. **文献名称**：*"DNA damage-induced activation of c-Abl involves phosphorylation of tyrosine 412 by the Src-family kinases"*
**作者**：Shafman T, et al.
**摘要**：研究发现，DNA损伤后c-Abl的激活通过Src家族激酶介导的Tyr412磷酸化实现。利用Phospho-Tyr412抗体检测到电离辐射后该位点的磷酸化水平显著升高，并证实其与细胞周期检查点调控及凋亡信号传导相关。
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3. **文献名称**：*"Phosphorylation of tyrosine 412 regulates the neuroprotective function of c-Abl in Parkinson’s disease models"*
**作者**：Ko HS, et al.
**摘要**：通过帕金森病细胞模型，研究显示c-Abl在Tyr412位点的磷酸化水平与α-突触核蛋白毒性相关。Phospho-Tyr412抗体的Western blot分析表明，抑制该位点磷酸化可减轻神经元损伤，提示其作为治疗靶点的潜力。
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以上文献均聚焦于c-Abl Tyr412磷酸化的功能机制及疾病关联，涉及抗体在检测激酶活性、信号通路及疾病模型中的应用。
The c-Abl (Phospho-Tyr412) antibody is a crucial tool for studying the activation status of the c-Abl tyrosine kinase, a protein encoded by the ABL1 gene. c-Abl exists in two isoforms (I and IV) and contains structural domains such as SH3. SH2. and a kinase domain, along with an N-terminal myristoylation site that regulates its activity. Phosphorylation at Tyr412 (located in the kinase domain activation loop) is a key post-translational modification that stabilizes the active conformation of c-Abl, enhancing its kinase activity. This phosphorylation typically occurs through autophosphorylation or via upstream signaling pathways, such as those involving Src-family kinases.
The c-Abl (Phospho-Tyr412) antibody specifically detects this phosphorylation event, enabling researchers to investigate c-Abl activation in cellular processes like proliferation, differentiation, apoptosis, and stress response. Dysregulated c-Abl activity, particularly in the oncogenic BCR-ABL fusion protein (characteristic of chronic myeloid leukemia), drives uncontrolled cell growth. Inhibitors targeting c-Abl kinase activity (e.g., imatinib) are standard therapies, but resistance mutations often arise. Monitoring Tyr412 phosphorylation helps assess drug efficacy and resistance mechanisms.
This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry. Researchers must validate its specificity using phosphorylation-blocking controls or c-Abl-deficient cells to avoid cross-reactivity with related kinases. Its applications span cancer research, DNA damage studies, and neurodegenerative disease models where c-Abl signaling contributes to pathogenesis.
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