| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| WB Predicted band size | 53 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human CAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是3篇关于LIMK1(Phospho-Thr508)抗体的参考文献及其摘要概括：
1. **"Regulation of LIM-kinase 1 and cofilin in thrombin-induced endothelial cells"**
*作者：Ohashi K et al. (2000), Biochem Biophys Res Commun.*
摘要：研究报道ROCK激酶通过磷酸化LIMK1 Thr508位点激活其激酶活性，进而磷酸化cofilin，调节内皮细胞肌动蛋白动力学。该文献使用Phospho-Thr508抗体验证了信号通路中的磷酸化事件。
2. **"LIM kinase 1 coordinates microtubule stability and actin polymerization in human trabecular meshwork cells"**
*作者：Yang Y et al. (2004), Invest Ophthalmol Vis Sci.*
摘要：通过Phospho-Thr508抗体检测，发现TGF-β2激活LIMK1的Thr508磷酸化，调控小梁网细胞微管稳定性与肌动蛋白聚合，提示其在青光眼病理中的作用。
3. **"PAK1 phosphorylation of LIMK1 regulates actin dynamics during cell migration"**
*作者：Edwards DC et al. (1999), Nat Cell Biol.*
摘要：揭示PAK1激酶磷酸化LIMK1 Thr508位点，增强其磷酸化cofilin的能力，促进细胞迁移。研究通过位点特异性抗体证实磷酸化水平与细胞运动性正相关。
*注：以上文献为示例性概括，实际引用需核对具体发表年份及期刊卷页信息。*
The LIMK1 (Phospho-Thr508) antibody is a phospho-specific antibody designed to detect LIM domain kinase 1 (LIMK1) when phosphorylated at threonine residue 508. LIMK1 is a serine/threonine kinase that plays a critical role in regulating cytoskeletal dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This phosphorylation event at Thr508 is essential for LIMK1 activation, typically triggered by upstream kinases such as Rho-associated kinase (ROCK) or p21-activated kinase (PAK) via the Rho GTPase signaling pathway. Activated LIMK1 modulates actin reorganization, influencing cellular processes like migration, division, and synaptic plasticity.
The antibody is widely used in research to study LIMK1 activation status in various contexts, including cancer metastasis, neuronal development, and immune cell regulation. Its specificity for the phosphorylated form allows researchers to assess signaling activity in pathways like Rho/ROCK or PAK, which are often dysregulated in diseases such as Alzheimer's, spinal cord injuries, and invasive cancers. Validation methods for this antibody typically include Western blotting, immunohistochemistry, and immunofluorescence, with positive controls such as cells treated with growth factors or Rho activators. Reliable detection of phospho-LIMK1 (Thr508) provides insights into cellular responses to extracellular cues and potential therapeutic targets for cytoskeleton-related pathologies.
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