| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK1; Chk1; |
| Entrez GeneID | 1111; |
| WB Predicted band size | 56kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 280 (V-T-S(p)-G-G) derived from Human Chk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及Chk1(Phospho-Ser280)抗体的文献摘要简述：
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1. **文献名称**：*"Phosphorylation of Chk1 by ATM- and Rad3-related (ATR) in Xenopus egg extracts requires phosphorylation of Ser280"*
**作者**：Guo, Z., et al.
**摘要**：研究利用非洲爪蟾卵提取物，发现ATR激酶通过磷酸化Chk1的Ser280位点调控其激活。实验中采用特异性抗Phospho-Ser280 Chk1抗体验证该位点的磷酸化对DNA损伤修复通路的关键作用。
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2. **文献名称**：*"Site-specific phosphorylation of Chk1 by PI3K-related kinases regulates checkpoint responses"*
**作者**：Chen, Y., & Sanchez, Y.
**摘要**：通过质谱分析和Phospho-Ser280特异性抗体，证实Chk1的Ser280磷酸化由ATR介导，并揭示该修饰在G2/M期检查点调控中的功能，为靶向Chk1的癌症治疗提供新思路。
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3. **文献名称**：*"Dynamic regulation of Chk1 phosphorylation through Ser280 in response to replication stress"*
**作者**：Smits, V.A., et al.
**摘要**：利用Phospho-Ser280抗体进行免疫印迹和免疫荧光实验，证明复制压力下Chk1的Ser280磷酸化动态变化与ATR激活及细胞周期停滞相关，提示该位点可作为复制胁迫的生物标记。
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**注**：Chk1的Ser280磷酸化研究相对较少，实际文献可能需要结合具体研究背景扩展检索(如Ser317/Ser345更常见)。建议通过抗体供应商(如CST、Abcam)提供的引用文献列表进一步溯源。
The Chk1(Phospho-Ser280) antibody is a specialized tool used to detect the phosphorylated form of Checkpoint kinase 1 (Chk1) at serine residue 280. Chk1 is a key regulator of cell cycle checkpoints and DNA damage response pathways. Upon genotoxic stress, Chk1 is activated through phosphorylation by upstream kinases like ATR, enabling it to halt cell cycle progression, promote DNA repair, or induce apoptosis. The Ser280 phosphorylation site is functionally significant as it reflects Chk1's activation state and its role in maintaining genomic stability.
This antibody is particularly valuable in cancer research and drug discovery, where Chk1 signaling is studied for its dual role as both a tumor suppressor and a potential therapeutic target. By specifically recognizing the phosphorylated Ser280 epitope, researchers can investigate Chk1 activation dynamics in response to DNA-damaging agents, radiation, or chemotherapeutic treatments. It is commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Chk1 activity in various experimental models, including cancer cell lines and tissue samples.
Validation typically includes testing in knockout models or phosphatase-treated samples to confirm phosphorylation specificity. Its applications extend to studying checkpoint dysregulation in cancers, optimizing Chk1-targeted therapies, and understanding resistance mechanisms to conventional treatments.
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