| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | FOXO1 |
| Entrez GeneID | 2308; |
| WB Predicted band size | 78-82 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 319 (T-S-S(p)-N-A) derived from Human FKHR/FOXO1A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **FKHR (Phospho-Ser319) 抗体** 的参考文献示例(注：以下为假设性文献，实际引用时请核实来源)：
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1. **"AKT-mediated phosphorylation of FOXO1 at Ser319 regulates cellular proliferation"**
- **作者**: Nakamura M, et al.
- **摘要**: 研究揭示了AKT激酶通过磷酸化FOXO1的Ser319位点，促进其从细胞核转运至胞质，从而抑制凋亡并增强细胞增殖。该文献使用Phospho-Ser319抗体进行Western blot和免疫荧光分析。
2. **"Phosphorylation of FOXO1 (Ser319) modulates glucose metabolism in diabetic models"**
- **作者**: Cheng Z, et al.
- **摘要**: 探讨胰岛素信号通过磷酸化FOXO1(Ser319)抑制肝脏糖异生的机制，利用Phospho-Ser319抗体在小鼠模型中验证FOXO1的磷酸化水平与血糖调控的关系。
3. **"FOXO1 phosphorylation at Ser319 enhances chemoresistance in colorectal cancer"**
- **作者**: Wang Y, et al.
- **摘要**: 分析结直肠癌细胞中化疗药物诱导的FOXO1磷酸化(Ser319)对耐药性的影响，通过该抗体进行免疫组化检测，发现磷酸化水平与患者预后负相关。
4. **"Dynamic regulation of FOXO1 subcellular localization by Ser319 phosphorylation"**
- **作者**: Brunet A, et al.
- **摘要**: 研究生长因子刺激下FOXO1的磷酸化动力学，证实Ser319位点的修饰通过14-3-3蛋白结合调控其核质穿梭，Phospho-Ser319抗体用于流式细胞术和活细胞成像。
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**注意**：以上文献名为示例，实际研究中请通过PubMed、Google Scholar等平台检索关键词 **"FOXO1 Phospho-Ser319"** 或 **"FKHR Ser319 phosphorylation"** 获取真实文献。
The FKHR (Phospho-Ser319) antibody is designed to detect Forkhead Box Protein O1 (FOXO1) when phosphorylated at serine residue 319. FOXO1. also known as FKHR (Forkhead in Rhabdomyosarcoma), is a transcription factor critical for regulating cellular processes like apoptosis, cell cycle arrest, oxidative stress response, and glucose metabolism. Its activity is tightly controlled by post-translational modifications, particularly phosphorylation. Phosphorylation at Ser319. along with adjacent residues (e.g., Ser256. Ser253), is mediated by upstream kinases such as AKT (Protein Kinase B) in response to insulin or growth factor signaling. This modification promotes FOXO1 nuclear exclusion, binding to 14-3-3 proteins, and subsequent cytoplasmic retention, effectively inhibiting its transcriptional activity.
The FKHR (Phospho-Ser319) antibody is widely used in research to study FOXO1 regulation in pathways linked to cancer, diabetes, and aging. It enables detection of phosphorylated FOXO1 via techniques like Western blotting, immunofluorescence, or immunohistochemistry, providing insights into pathway activation status. Specificity for the phosphorylated Ser319 epitope makes it valuable for distinguishing active versus inactive FOXO1 in cellular or tissue samples. Studies using this antibody have elucidated FOXO1's role in tumor suppression, metabolic disorders, and response to therapeutic agents targeting PI3K/AKT signaling. Proper validation with phosphorylation-deficient mutants or kinase inhibitors is recommended to confirm signal specificity.
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