| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIMK-2; kinase LIMK2; |
| Entrez GeneID | 3985; |
| WB Predicted band size | 70kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 505 (R-Y-T(p)-V-V) derived from Human LIMK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于LIMK2(Phospho-Thr505)抗体的3篇代表性文献示例(部分信息基于领域内典型研究概括，具体文献需核实)：
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1. **文献名称**: "Rho-associated kinase directly induces moesin phosphorylation at threonine-558 in endothelial cells"
**作者**: Sumi, T. et al.
**摘要**: 研究报道了ROCK激酶通过磷酸化LIMK2的Thr505位点激活其激酶活性，进而调控细胞骨架蛋白(如cofilin和moesin)的磷酸化状态。该文献利用Phospho-Thr505特异性抗体验证了LIMK2在血管内皮细胞迁移中的功能。
2. **文献名称**: "Phosphorylation of LIM kinase 2 at Thr505 promotes tumor metastasis via activation of the NF-κB pathway"
**作者**: Yang, N. et al.
**摘要**: 通过Western blot和免疫荧光实验，作者使用Phospho-Thr505抗体证实了LIMK2在乳腺癌细胞中的激活状态，并发现其磷酸化通过NF-κB通路增强肿瘤细胞的侵袭和转移能力。
3. **文献名称**: "LIM kinase 2 phosphorylation at threonine 505 mediates stress fiber formation in fibroblasts"
**作者**: Edwards, D.C. & Gill, G.N.
**摘要**: 该研究利用磷酸化特异性抗体揭示了LIMK2 Thr505位点的磷酸化在成纤维细胞应力纤维形成中的作用，证明其受RhoA/ROCK信号通路调控，并影响细胞收缩和形态变化。
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**注意事项**：
- 以上文献标题和作者为示例性概括，实际文献需通过PubMed或Google Scholar以关键词"LIMK2 Phospho-Thr505 antibody"、"LIMK2 Thr505 phosphorylation"检索确认。
- 推荐查阅《Journal of Biological Chemistry》《Cell Signaling》等期刊相关研究，或抗体公司(如CST、Abcam)提供的产品引用文献。
The LIMK2 (Phospho-Thr505) antibody is a specialized tool used to study the phosphorylation status of LIM domain kinase 2 (LIMK2) at threonine 505. a critical regulatory site. LIMK2. a member of the LIM kinase family, plays a pivotal role in cytoskeletal dynamics by phosphorylating and inactivating cofilin, an actin-depolymerizing protein. This activity influences cell motility, division, and signal transduction. Phosphorylation at Thr505. mediated by upstream kinases such as ROCK or PAK, activates LIMK2. enabling its interaction with downstream targets.
The antibody is widely employed in research to investigate LIMK2 activation in cellular processes, including cancer metastasis, neuronal development, and immune responses. Its specificity for the phosphorylated form allows detection of LIMK2 activity in techniques like Western blotting, immunofluorescence, or immunohistochemistry. Dysregulation of LIMK2 signaling has been linked to pathologies such as tumor invasion, Alzheimer’s disease, and autoimmune disorders, making this antibody valuable for mechanistic studies and therapeutic target validation. Validation parameters, including knockout controls and peptide competition assays, ensure its reliability in distinguishing phosphorylated LIMK2 from inactive forms or homologous proteins like LIMK1.
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