| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | kinase Cdk2; p33 protein kinase; |
| Entrez GeneID | 1017; |
| WB Predicted band size | 34kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 160 (T-Y-T(p)-H-E) derived from Human CDK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CDK2(Phospho-Thr160)抗体的3篇参考文献示例(文献信息为概括性描述，非真实引用)：
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1. **文献名称**: *"Cyclin-dependent kinase 2 (Cdk2) phosphorylation at Thr160 regulates its activity during G1/S progression"*
**作者**: Rosenblatt, J. et al.
**摘要**: 该研究通过使用Phospho-Thr160特异性抗体，证实CDK2在G1/S期转换过程中因Thr160位点的磷酸化而被激活，并揭示了其与Cyclin E结合的依赖性。
2. **文献名称**: *"Mechanisms of CDK activation: Role of CAK phosphorylation and regulatory subunit binding"*
**作者**: Harper, J.W., Elledge, S.J.
**摘要**: 文章利用Phospho-Thr160抗体进行免疫印迹分析，阐明了CDK2的Thr160磷酸化由CAK(CDK激活激酶)介导，并证明该修饰对CDK2的酶活性和细胞周期进程至关重要。
3. **文献名称**: *"Deregulation of CDK2 phosphorylation in human cancer cells correlates with loss of cell cycle control"*
**作者**: Toyoshima, H., Hunter, T.
**摘要**: 通过Phospho-Thr160抗体检测多种肿瘤细胞系，研究发现CDK2的异常磷酸化与细胞周期失控及肿瘤增殖相关，提示其作为癌症治疗靶点的潜力。
4. **文献名称**: *"The Cell Cycle: Principles of Control"*(相关章节)
**作者**: Morgan, D.O.
**摘要**: 该书籍章节讨论了CDK2的磷酸化调控机制，引用Phospho-Thr160抗体的实验数据，说明Thr160磷酸化是CDK2与Cyclin A结合后构象变化的关键步骤。
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注：以上文献名为示例性描述，实际引用需根据具体研究查找真实文献(如PubMed等数据库)。
The CDK2 (Phospho-Thr160) antibody is a specialized tool used to detect the activated form of Cyclin-Dependent Kinase 2 (CDK2) phosphorylated at threonine 160. CDK2 is a serine/threonine kinase critical for cell cycle progression, particularly during the G1/S transition and S phase. Its activity depends on binding to cyclins (E or A) and phosphorylation at Thr160. a residue located in the kinase's T-loop. This phosphorylation event, mediated by the CDK-activating kinase (CAK), induces conformational changes that enhance catalytic activity and promote substrate recognition. Dysregulation of CDK2 or its phosphorylation status is linked to uncontrolled cell proliferation, cancer, and other diseases.
The CDK2 (Phospho-Thr160) antibody enables researchers to study CDK2 activation dynamics in response to cellular signals, drug treatments, or disease states. It is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to assess cell cycle regulation, DNA replication, and checkpoint control. By specifically targeting the phosphorylated form, this antibody helps distinguish active CDK2 from its inactive counterparts, providing insights into mechanisms underlying oncogenesis or therapeutic interventions targeting cell cycle kinases. Its applications extend to basic research, drug discovery, and biomarker studies in oncology.
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