Rabbit Polyclonal Src(Phospho-Tyr529) Antibody

The Src (Phospho-Tyr529) antibody is designed to detect the phosphorylated form of tyrosine residue 529 (Tyr529) in the Src protein, a non-receptor tyrosine kinase involved in regulating cell proliferation, differentiation, adhesion, and survival. Src activity is tightly controlled by phosphorylation at specific residues. Phosphorylation at Tyr529 (corresponding to Tyr530 in human Src due to species-specific numbering) by C-terminal Src kinase (CSK) induces a closed, inactive conformation by promoting intramolecular interactions between the SH2 domain and the phosphorylated tail. This inhibits Src kinase activity and downstream signaling. Conversely, dephosphorylation of Tyr529 by protein tyrosine phosphatases activates Src, enabling its participation in oncogenic pathways when dysregulated. The Src (Phospho-Tyr529) antibody is widely used in cancer research, as hyperactivation of Src through reduced phosphorylation at this site is linked to tumor progression, metastasis, and drug resistance. It serves as a critical tool for studying Src regulatory mechanisms, signaling crosstalk, and therapeutic targeting in diseases like breast, colon, and pancreatic cancers. Specificity for the phosphorylated Tyr529 ensures accurate detection of Src inactivation status in applications such as Western blotting, immunohistochemistry, and flow cytometry.