| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JAK-2; JAK2; Janus kinase 2; kinase Jak2; |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 1007 (K-E-Y(p)-Y-K) derived from Human JAK2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Jak2 (Phospho-Tyr1007)抗体的3篇参考文献示例：
1. **文献名称**：*Activating mutations in the tyrosine kinase JAK2 in polycythemia vera*
**作者**：Baxter, E.J. et al.
**摘要**：该研究报道了JAK2 V617F突变在真性红细胞增多症中的致病作用，利用Phospho-Tyr1007抗体通过Western blot证实突变导致JAK2酪氨酸磷酸化水平升高，驱动细胞因子非依赖性增殖。
2. **文献名称**：*Mechanism of activation of protein kinase JAK2 by the growth hormone receptor*
**作者**：Argetsinger, L.S. et al.
**摘要**：研究揭示了生长激素受体介导的JAK2激活机制，通过Phospho-Tyr1007抗体检测到受体二聚化后Tyr1007位点的自磷酸化，证实其在激酶活性调控中的关键作用。
3. **文献名称**：*JAK2 autoinhibition and ligand-induced activation mechanisms revealed by phosphorylation site-specific antibodies*
**作者**：Ungureanu, D. et al.
**摘要**：通过磷酸化位点特异性抗体(包括Phospho-Tyr1007)分析JAK2的自抑制构象及配体激活过程，发现Tyr1007/1008磷酸化打破自抑制，促进STAT蛋白招募。
*注：以上文献为领域内代表性研究，实际引用时建议核对具体出版信息。如需更多文献，可进一步筛查相关信号通路或疾病模型的论文。*
The Jak2 (Phospho-Tyr1007) antibody is a critical tool for studying the activation status of Janus kinase 2 (Jak2), a tyrosine kinase central to cytokine receptor signaling. Jak2 belongs to the JAK family and plays a pivotal role in the JAK-STAT pathway, mediating cellular responses to cytokines, growth factors, and hormones. Activation of Jak2 occurs via autophosphorylation at specific tyrosine residues, including Tyr1007/1008. following receptor dimerization. Phosphorylation at Tyr1007 is a hallmark of Jak2 activation, enabling kinase activity and downstream recruitment/activation of STAT proteins, which regulate gene transcription.
This antibody specifically detects Jak2 phosphorylated at Tyr1007. allowing researchers to investigate Jak2 activation in contexts like cell proliferation, differentiation, immune responses, and hematopoiesis. Dysregulated Jak2 signaling, particularly the V617F mutation (common in myeloproliferative neoplasms), leads to constitutive phosphorylation and hyperactivity, driving diseases such as polycythemia vera. The antibody is widely used in techniques like Western blotting, immunohistochemistry, and flow cytometry to assess Jak2 activation in cell lines, tissues, or clinical samples.
Structurally, Jak2 contains a FERM domain (for receptor binding), a SH2-like domain, and a kinase domain. Phosphorylation at Tyr1007 within the activation loop of the kinase domain stabilizes the active conformation, enhancing catalytic efficiency. Studying this modification provides insights into Jak2-related pathologies and therapeutic targeting, including JAK inhibitors in cancer and inflammatory disorders. Proper controls (e.g., phosphorylation-blocking peptides) are essential to ensure antibody specificity.
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