| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VAV |
| Entrez GeneID | 7409; |
| WB Predicted band size | 95kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Rat |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 174 (E-I-Y(p)-E-D) derived from Human Vav. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Vav(Phospho-Tyr174)抗体的3篇参考文献示例，包含文献名称、作者及摘要概要：
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1. **文献名称**：*"Phosphorylation of Tyr174 in Vav1 Regulates T-cell Receptor-induced Actin Cytoskeleton Dynamics"*
**作者**：K. Miletic et al.
**摘要**：本研究揭示了T细胞受体(TCR)激活后，Vav1在Tyr174位点的磷酸化对其鸟苷酸交换因子(GEF)活性的调控作用。通过使用特异性Phospho-Tyr174抗体进行Western blot分析，发现该位点的磷酸化是Vav1介导Rac1激活和细胞骨架重组的必要条件，对T细胞免疫突触形成至关重要。
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2. **文献名称**：*"Vav1 Phosphorylation at Tyr174 Links B-cell Receptor Signaling to NF-κB Activation"*
**作者**：S. Watanabe et al.
**摘要**：文章通过Phospho-Tyr174抗体验证了B细胞受体(BCR)刺激后Vav1的磷酸化动力学，并证明该修饰通过PI3K/Akt通路促进NF-κB活化。抗体特异性在敲除Vav1的细胞模型中得到验证，为BCR信号转导机制提供了新见解。
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3. **文献名称**：*"Development of a Phosphospecific Antibody for Vav1 Tyr174 and Its Application in Chronic Lymphocytic Leukemia"*
**作者**：L. Fernández et al.
**摘要**：本研究报道了一种针对Vav1 Tyr174磷酸化位点的兔单克隆抗体的开发与验证。通过免疫沉淀和质谱分析确认其特异性，并应用于慢性淋巴细胞白血病(CLL)患者样本，发现高磷酸化水平与疾病进展及耐药性相关。
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**备注**：以上文献为示例，实际引用时需核实数据库(如PubMed、Google Scholar)中的真实文献，并补充完整期刊信息及DOI号。若需进一步扩展，可关注信号通路、癌症或免疫学领域的高影响力期刊(如*Nature Immunology*、*Blood*等)。
The Vav family of proteins (Vav1. Vav2. Vav3) are guanine nucleotide exchange factors (GEFs) that activate Rho/Rac GTPases, playing critical roles in cellular signaling, cytoskeletal reorganization, and immune cell activation. Vav1. predominantly expressed in hematopoietic cells, is a key regulator of antigen receptor signaling in lymphocytes. Its activity is tightly controlled by phosphorylation at specific tyrosine residues. Phosphorylation at Tyr174 (human Vav1. corresponding to Tyr142 in mouse) occurs within the acidic domain, a regulatory region critical for autoinhibition. This post-translational modification, mediated by Src-family kinases (e.g., Lck, Fyn) upon receptor engagement, relieves autoinhibitory interactions, enabling GEF activity and downstream signaling.
The Vav (Phospho-Tyr174) antibody specifically detects this phosphorylation event, serving as a crucial tool for studying Vav1 activation dynamics. Researchers employ it to investigate immune receptor signaling (e.g., TCR, BCR), cellular motility, and oncogenic pathways, as aberrant Vav activation is linked to malignancies like leukemia and lymphoma. Applications include Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation status in response to stimuli (e.g., antigen exposure, growth factors). Validated in multiple studies, this antibody aids in deciphering mechanisms underlying immune regulation, cancer progression, and potential therapeutic targeting of Vav-dependent pathways.
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