Rabbit Polyclonal SP1(Phospho-Thr739) Antibody

The SP1 (Phospho-Thr739) antibody is a phosphospecific antibody designed to detect the transcription factor SP1 (Specificity Protein 1) when phosphorylated at threonine residue 739. SP1. a member of the SP/KLF family, regulates the expression of numerous genes by binding GC-rich promoter elements. Post-translational modifications, including phosphorylation, modulate its transcriptional activity, stability, and interactions with co-regulators. Phosphorylation at Thr739 is implicated in altering SP1’s DNA-binding affinity or subcellular localization, potentially influencing its role in cell proliferation, apoptosis, and differentiation. This site-specific phosphorylation may be mediated by kinases such as ERK or CDK, linking SP1 activity to signaling pathways like MAPK or cell cycle regulation. The SP1 (Phospho-Thr739) antibody is widely used in Western blotting, immunofluorescence, and ChIP assays to study SP1’s phosphorylation status in contexts such as cancer, where dysregulated SP1 activity contributes to oncogenesis. Its specificity is validated using knockout controls or phosphatase-treated samples. Research utilizing this antibody has advanced understanding of SP1’s regulatory mechanisms in diseases, offering insights into therapeutic targeting of phosphorylation-dependent SP1 functions.