| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KS6B1; P70-S6K; RPS6KB1; S6K; |
| Entrez GeneID | 6198; |
| WB Predicted band size | 70 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 424 (P-V-S(p)-P-V) derived from Human p70 S6 Kinase. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及p70S6 Kinase (Phospho-Ser424)抗体的代表性文献摘要：
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1. **"Ribosomal protein S6 phosphorylation: from protein synthesis to cell size"**
*作者：Ruvinsky I, et al. (Trends Biochem Sci, 2006)*
摘要：探讨p70S6K在mTOR信号通路中通过Ser424等位点的磷酸化调控核糖体蛋白S6的机制，分析其对细胞生长和周期的影响，并验证抗体在检测磷酸化状态中的应用。
2. **"Regulation and function of the mTOR kinase in human disease"**
*作者：Magnuson B, et al. (Nat Rev Mol Cell Biol, 2012)*
摘要：综述mTORC1/2复合物对p70S6K的磷酸化调控，强调Ser424位点作为下游靶点的功能及其在癌症和代谢疾病中的作用，引用该抗体用于临床前模型研究。
3. **"The role of the PI3K-Akt-mTOR pathway in human cancer"**
*作者：Pearce LR, et al. (Oncogene, 2010)*
摘要：分析p70S6K在肿瘤中的异常激活机制，通过磷酸化特异性抗体(包括Ser424)揭示其与PI3K/Akt信号串扰的关系，为靶向治疗提供依据。
4. **"Phosphorylation of p70S6 kinase in vivo is mediated by the mTOR-dependent phosphorylation of Ser424"**
*作者：Pende M, et al. (J Biol Chem, 2000)*
摘要：通过体内实验验证mTOR依赖的Ser424磷酸化对p70S6K活性的调控，使用特异性抗体证明该位点磷酸化与细胞增殖的直接关联。
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以上文献覆盖了该抗体的应用场景(如信号通路分析、疾病模型研究)及Ser424位点的功能机制。如需具体实验细节，建议进一步检索PubMed或抗体厂商提供的引用文献列表。
The p70 S6 Kinase (p70S6K), a serine/threonine kinase, plays a critical role in the mTOR (mammalian target of rapamycin) signaling pathway, regulating cell growth, proliferation, and metabolism by phosphorylating the S6 ribosomal protein to promote protein synthesis. Phosphorylation at specific residues, such as Ser424. is essential for its activation and downstream signaling. The Phospho-Ser424 modification occurs in the autoinhibitory domain of p70S6K, relieving intramolecular repression and enabling kinase activity. This phosphorylation event is mediated by upstream kinases, including phosphoinositide-dependent kinase-1 (PDK1) and mTOR complex 1 (mTORC1), in response to growth factors, nutrients, or insulin.
Anti-p70S6K (Phospho-Ser424) antibodies are widely used in biomedical research to study mTOR pathway activation under physiological or pathological conditions, such as cancer, metabolic disorders, or neurodegenerative diseases. These antibodies enable the detection and quantification of phosphorylated p70S6K via techniques like Western blotting, immunofluorescence, or immunohistochemistry. Specificity for the Ser424-phosphorylated form ensures accurate assessment of kinase activation status, distinguishing it from inactive or differentially phosphorylated isoforms. Validated antibodies are critical for exploring therapeutic targeting of mTOR signaling and understanding mechanisms underlying diseases linked to dysregulated cell growth and survival.
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