Rabbit Polyclonal IKK-β(Phospho-Tyr199) Antibody

The IKK-β (Phospho-Tyr199) antibody is a crucial tool for studying the regulation and activation of the inhibitor of nuclear factor kappa-B kinase subunit beta (IKK-β), a central component of the IKK complex within the NF-κB signaling pathway. IKK-β plays a pivotal role in phosphorylating IκB proteins, leading to their degradation and subsequent activation of NF-κB transcription factors, which regulate immune responses, inflammation, and cell survival. Phosphorylation at specific residues, such as Tyr199. is critical for modulating IKK-β activity. While IKK-β is traditionally activated via serine phosphorylation (e.g., Ser177/181), emerging evidence suggests tyrosine phosphorylation (e.g., Tyr199) may fine-tune its function or mediate cross-talk with other signaling pathways, such as those involving growth factors or stress responses. This antibody specifically detects IKK-β when phosphorylated at Tyr199. enabling researchers to investigate post-translational modifications under various physiological or pathological conditions, including inflammation, cancer, or autoimmune diseases. It is widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess activation states, cellular localization, or interactions in response to stimuli like cytokines, pathogens, or inhibitors. Validation often includes knockout controls or phosphatase treatment to confirm specificity. Proper handling of samples (e.g., protease/phosphatase inhibitors) is essential to preserve phosphorylation signals.