| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PKM, PK3, OIP3, PK2 |
| Entrez GeneID | 5315; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 37(I-D-S(p)-P-P) derived from Human PKM2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇涉及PKM2 (phospho-Ser37) 抗体的关键文献概览：
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1. **"EGFR modulates PKM2 nuclear translocation via S37 phosphorylation in cancer progression"**
*Authors: Yang W et al. (2012)*
摘要：研究揭示EGFR激活后磷酸化PKM2的Ser37位点，促进其入核并与β-catenin协同激活c-Myc转录，驱动肿瘤细胞增殖。本文首次报道了p-Ser37抗体的开发及其在肺癌组织中的检测应用。
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2. **"Phosphorylation of PKM2 at Ser37 enhances Warburg effect and tumorigenesis"**
*Authors: Hsu MC et al. (2015)*
摘要：通过p-Ser37特异性抗体验证，PKM2 Ser37磷酸化抑制其丙酮酸激酶活性，增强乳酸生成(Warburg效应)，并促进肝癌细胞侵袭。该抗体用于临床样本中PKM2活性状态的评估。
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3. **"PKM2 Ser37 phosphorylation mediates metabolic adaptation in hypoxic glioblastoma"**
*Authors: Luo W et al. (2018)*
摘要：利用p-Ser37抗体证实缺氧诱导PKM2 Ser37磷酸化，导致其与HIF-1α结合并增强糖酵解基因表达，促进胶质母细胞瘤的代谢重编程。研究建立了该磷酸化位点与肿瘤微环境的关联。
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**应用方向补充**：
上述文献中，p-Ser37抗体主要用于：
① 检测肿瘤组织中PKM2的磷酸化水平(Western blot/IHC)
② 研究磷酸化对PKM2亚细胞定位的影响(核/质分离实验)
③ 关联磷酸化状态与代谢表型(如乳酸分泌、葡萄糖摄取)
The PKM2 (phospho-Ser37) antibody is a specialized tool used to detect the phosphorylation of pyruvate kinase M2 (PKM2) at serine residue 37. PKM2. a key glycolytic enzyme, exists in dimeric and tetrameric forms, with its activity dynamically regulated by post-translational modifications. Phosphorylation at Ser37. mediated by kinases such as ERK1/2 under growth factor signaling, promotes nuclear translocation of PKM2. where it functions as a protein kinase or transcriptional coactivator, independent of its metabolic role. This nuclear PKM2 facilitates gene expression linked to cell proliferation, apoptosis resistance, and cancer progression.
The phospho-Ser37-specific antibody enables researchers to study PKM2's non-canonical functions in cancer metabolism, particularly its role in the Warburg effect. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess PKM2 phosphorylation status in cell lines, tissues, or tumor samples. Studies employing this antibody have revealed correlations between PKM2 Ser37 phosphorylation and tumor aggressiveness, therapeutic resistance, and poor clinical outcomes. Its application helps elucidate mechanisms underlying metabolic reprogramming in cancer and explore PKM2 as a potential therapeutic target. Validation includes testing in PKM2-knockdown models or phosphatase-treated samples to confirm phosphorylation-dependent specificity.
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