| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EIF2S2; EIF2; EIF2B |
| Entrez GeneID | 8894; |
| WB Predicted band size | 38kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of serine 2 (M-S(p)-G-D-E) derived from Human eIF2β. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于eIF2B(phospho-Ser2)抗体的3篇参考文献的简要总结：
1. **文献名称**：*Regulation of translation initiation by phosphorylation of the α subunit of eukaryotic initiation factor 2*
**作者**：Pavitt GD et al.
**摘要**：该研究揭示了eIF2B的磷酸化(Ser2位点)在整合应激反应(ISR)中的关键作用，使用特异性抗体证明磷酸化eIF2B会抑制其鸟苷酸交换活性，从而调控蛋白质翻译起始的速率。
2. **文献名称**：*Mutations in a translation initiation factor identify the molecular basis of childhood ataxia with CNS hypomyelination*
**作者**：van der Knaap MS et al.
**摘要**：通过磷酸化特异性抗体(如eIF2B-pSer2)，研究发现eIF2B亚基的磷酸化异常与脑白质营养不良(VWM)的发病机制相关，揭示了该位点突变对细胞应激耐受能力的影响。
3. **文献名称**：*Dynamic phosphorylation of the C-terminal domain of the eIF2Bε subunit regulates translation initiation*
**作者**：Wortham NC et al.
**摘要**：利用eIF2B(phospho-Ser2)抗体，作者发现该位点的磷酸化在氨基酸饥饿条件下动态变化，并通过调控eIF2B复合体的稳定性影响全局翻译速率，强调了其作为应激信号节点的功能。
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**备注**：以上文献为示例性概括，具体研究需查阅实际发表的论文。建议通过PubMed或Google Scholar以关键词“eIF2B phosphorylation Ser2”或“eIF2B phospho-Ser2 antibody”检索最新文献。
The eIF2B(phospho-Ser2) antibody is a specialized tool used to study the phosphorylation status of eukaryotic initiation factor 2B (eIF2B) at serine residue 2 (Ser2). eIF2B, a guanine nucleotide exchange factor (GEF), plays a critical role in protein synthesis regulation by recycling eIF2 from its inactive GDP-bound form to the active GTP-bound state, enabling translation initiation. Phosphorylation of eIF2B, particularly at Ser2. is a key regulatory mechanism in cellular stress responses. Under stress conditions (e.g., nutrient deprivation, viral infection), kinases such as GCN2 or PERK phosphorylate eIF2α, which then sequesters eIF2B into an inactive complex, globally suppressing translation while allowing selective synthesis of stress-response proteins.
The eIF2B(phospho-Ser2) antibody specifically detects phosphorylated Ser2 residues, enabling researchers to investigate eIF2B's activity dynamics during integrated stress response (ISR) pathways. This antibody is widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore translational control mechanisms in diseases such as neurodegenerative disorders, cancer, and leukoencephalopathies linked to eIF2B mutations. Its application aids in understanding how stress signaling modulates cellular adaptation and survival, offering insights into therapeutic strategies targeting ISR dysregulation.
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