Rabbit Polyclonal AKT1(phospho-Thr450) Antibody

The AKT1 (phospho-Thr450) antibody is a specialized tool used to detect the phosphorylation of threonine at position 450 (Thr450) in the AKT1 protein, a key serine/threonine kinase in the PI3K/AKT/mTOR signaling pathway. AKT1 regulates critical cellular processes such as survival, proliferation, and metabolism. Phosphorylation at Thr450 occurs constitutively during AKT1 synthesis, distinguishing it from activation-associated phosphorylation sites like Thr308 and Ser473. This modification is believed to stabilize the immature, inactive form of AKT1 by maintaining proper folding and preventing degradation until full maturation. Studies suggest Thr450 phosphorylation may influence AKT1's subcellular localization or interaction with chaperone proteins like HSP90. Antibodies targeting phospho-Thr450 are valuable for investigating AKT1's post-translational regulation, particularly in cancer research, as aberrant AKT signaling is linked to tumor progression and drug resistance. They enable detection of AKT1 maturation states via techniques like Western blotting or immunofluorescence. However, interpretation requires caution, as Thr450 phosphorylation status doesn't directly correlate with AKT1 enzymatic activity. These antibodies are often validated using phosphorylation-deficient mutants or phosphatase-treated samples to confirm specificity. Their application helps elucidate context-dependent AKT1 regulation in diseases, including diabetes and neurodegenerative disorders.