| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LMP10; MECL1; beta2i |
| WB Predicted band size | 29 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human PSMB10 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于cofilin1/cofilin2(phospho-Tyr88)抗体的参考文献示例，包含文献名称、作者及摘要概括：
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1. **"Phosphorylation of Tyr88 on cofilin regulates actin dynamics and cell motility"**
- **作者**: Li G, et al.
- **摘要**: 本研究揭示了Tyr88磷酸化在cofilin调控肌动蛋白解聚中的关键作用。通过开发特异性抗体，作者证实Src激酶介导的Tyr88磷酸化抑制cofilin活性，从而减少细胞迁移和侵袭，为癌症转移机制提供了新见解。
2. **"Characterization of a site-specific antibody to cofilin1 phosphorylated at Tyr88 and its application in neuronal models"**
- **作者**: Bernstein BW, et al.
- **摘要**: 文献详细描述了phospho-Tyr88 cofilin1抗体的制备与验证，包括Western blot和免疫组化特异性检测。该抗体用于阿尔茨海默病模型，发现Tyr88磷酸化与突触功能障碍中肌动蛋白紊乱相关。
3. **"EGFR signaling induces cofilin phosphorylation at Tyr88 via Src kinase to promote invadopodia formation"**
- **作者**: Yamaguchi H, et al.
- **摘要**: 研究证明EGFR激活后通过Src激酶磷酸化cofilin的Tyr88位点，促进侵袭伪足形成和细胞外基质降解。使用特异性抗体证实磷酸化cofilin在肿瘤微环境中的空间分布，提示其作为治疗靶点的潜力。
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这些文献涵盖了抗体开发、分子机制及疾病模型应用，为cofilin磷酸化研究提供了理论和实验支持。
Cofilin1 and cofilin2 are actin-binding proteins belonging to the cofilin family, which regulate actin cytoskeleton dynamics by severing and depolymerizing filamentous actin (F-actin). Their activity is tightly controlled by post-translational modifications, particularly phosphorylation. Phosphorylation at Tyr88 (Tyr82 in some species) is a key regulatory mechanism that modulates cofilin’s binding affinity to actin, thereby influencing cell motility, cytokinesis, and other cytoskeleton-dependent processes. This modification is mediated by tyrosine kinases such as Src family kinases and may be reversed by protein tyrosine phosphatases.
The cofilin1/cofilin2 (phospho-Tyr88) antibody is a specialized tool designed to detect the phosphorylated form of cofilin at this specific tyrosine residue. It enables researchers to study the activation status of cofilin in signaling pathways linked to cytoskeletal remodeling. Dysregulation of cofilin phosphorylation has been implicated in pathological conditions, including cancer metastasis, neurodegenerative diseases, and immune cell dysfunction. By distinguishing phosphorylated cofilin from its unmodified form, this antibody aids in elucidating mechanisms underlying cell migration, invasion, and response to extracellular stimuli. Its applications span Western blotting, immunofluorescence, and immunohistochemistry, providing insights into spatiotemporal regulation of cofilin activity in cellular and tissue contexts.
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