Rabbit Polyclonal CyclinE1(phospho-Thr395) Antibody

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     Western blot analysis of extracts from 293 cells untreated or treated with hydroxyurea using Cyclin E1(phospho-Thr395) Antibody P40325.    

Cyclin E1 is a key regulatory protein involved in cell cycle progression, particularly during the G1/S transition. It partners with cyclin-dependent kinase 2 (CDK2) to promote DNA replication and cell cycle entry. Post-translational modifications, such as phosphorylation, tightly regulate Cyclin E1 activity, stability, and subcellular localization. Phosphorylation at Thr395 is one such modification implicated in modulating its function. Studies suggest that Thr395 phosphorylation may influence Cyclin E1-CDK2 complex formation, protein stability, or degradation, though its precise mechanistic role remains under investigation. Dysregulation of Cyclin E1. including aberrant phosphorylation, is frequently observed in cancers and linked to uncontrolled proliferation, genomic instability, and poor prognosis.

The Cyclin E1 (phospho-Thr395) antibody is a specialized tool designed to detect Cyclin E1 specifically phosphorylated at Thr395. It enables researchers to study this modification in various contexts, such as cell cycle checkpoints, DNA damage responses, or oncogenic signaling. This antibody is commonly used in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation dynamics in cell lines, tissues, or disease models. Its applications extend to cancer research, where Cyclin E1 overexpression or dysregulated phosphorylation may serve as a biomarker or therapeutic target. Validation of antibody specificity, including knockout controls or peptide competition assays, is critical to ensure accurate interpretation of results.