Rabbit Polyclonal CDK6(phospho-Tyr13) Antibody

The CDK6 (phospho-Tyr13) antibody is a specialized tool used to detect the phosphorylation of cyclin-dependent kinase 6 (CDK6) at tyrosine residue 13. a post-translational modification implicated in regulating its activity. CDK6. a serine/threonine kinase, plays a critical role in cell cycle progression by forming complexes with D-type cyclins to drive G1-to-S phase transition. Phosphorylation at Tyr13 has been identified as a regulatory mechanism influencing CDK6 function, potentially modulating its interaction with binding partners, subcellular localization, or catalytic activity. Studies suggest that this modification may be linked to signaling pathways involving tyrosine kinases, such as those activated by growth factors or oncogenic stimuli, though its precise biological significance remains under investigation. The antibody is commonly employed in cancer research, as CDK6 dysregulation is associated with tumorigenesis and resistance to targeted therapies. Researchers utilize this reagent in techniques like Western blotting, immunofluorescence, or immunoprecipitation to study phosphorylation-dependent CDK6 behavior in cellular models or clinical samples. Its specificity for the phosphorylated epitope enables precise analysis of CDK6 activation states, contributing to mechanistic insights into cell cycle control and therapeutic targeting. Validation typically includes testing in phosphorylation-deficient mutants to confirm epitope recognition.