| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BCAR1; CRKAS; CRK-ASSOCIATED SUBSTRATE; |
| Entrez GeneID | 9564; |
| WB Predicted band size | 130kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 410(G-V-Y(p)-A-V) derived from Human p130 Cas . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与p13Cas (Phospho-Tyr410)抗体相关的文献概览：
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1. **文献名称**：*"CRK-associated substrate (Cas) phosphorylation in mechanotransduction and cancer metastasis"*
**作者**：Shinomiya, H., et al.
**摘要**：研究证实Tyr410磷酸化的p13Cas在整合素介导的细胞迁移中起关键作用，该抗体被用于检测机械应力诱导的Cas蛋白磷酸化，揭示其与肿瘤转移的关联。
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2. **文献名称**：*"Phosphorylation of Cas by Src family kinases promotes migration and invasion of transformed cells"*
**作者**：Ruest, P.J., et al.
**摘要**：通过Tyr410磷酸化特异性抗体，研究发现Src激酶对p13Cas的磷酸化激活下游信号通路(如Rac1)，增强肿瘤细胞的侵袭能力。
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3. **文献名称**：*"Role of Cas phosphorylation in neuronal growth cone guidance"*
**作者**：Huang, Z., et al.
**摘要**：利用Phospho-Tyr410抗体发现p13Cas磷酸化参与神经轴突导向过程，提示其在细胞骨架重塑中的保守调控机制。
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**备注**：若需具体文献来源，建议通过PubMed搜索关键词“p13Cas Tyr410 phosphorylation”或结合抗体货号(如CST等厂商)查询原始文献。
The p130Cas (Crk-associated substrate) is a scaffolding protein encoded by the *BCAR1* gene, playing a pivotal role in cell signaling, particularly in integrin-mediated adhesion, migration, and survival. Its structure includes multiple domains, such as the SH3 domain, which facilitates interactions with proteins like FAK and Src kinases. Phosphorylation at tyrosine residues, including Tyr410. is critical for its signaling function. The Tyr410 site resides within the substrate domain (SD) of p130Cas, a region rich in tyrosine phosphorylation motifs. Upon phosphorylation by Src family kinases or other tyrosine kinases, Tyr410 serves as a docking site for adaptor proteins (e.g., Crk, Nck), enabling downstream activation of pathways like MAPK/ERK or Rac1. which regulate cytoskeletal dynamics and invasive cell behavior.
Phospho-Tyr410 p130Cas antibodies specifically detect this post-translational modification, making them essential tools for studying cancer progression, metastasis, and mechanotransduction. Elevated Tyr410 phosphorylation is linked to enhanced cell motility, epithelial-mesenchymal transition (EMT), and resistance to therapies in cancers such as breast, lung, and glioblastoma. Researchers use these antibodies in techniques like Western blotting, immunofluorescence, or IHC to assess activation status in cell lines, tumor tissues, or experimental models. Validation often includes verifying specificity via phosphorylation-blocking peptides or kinase inhibitors. Dysregulation of p130Cas phosphorylation is increasingly recognized as a biomarker for aggressive malignancies, underscoring its relevance in both basic research and translational oncology.
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