Rabbit Polyclonal CD32(Phospho-Tyr292) Antibody

The CD32 (Phospho-Tyr292) antibody is designed to detect the phosphorylated form of CD32 (Fcγ receptor II, FcγRII) at tyrosine residue 292. a key post-translational modification involved in immune signaling. CD32. a member of the Fc receptor family, binds the Fc region of IgG antibodies and exists in multiple isoforms (e.g., FcγRIIA, FcγRIIB) expressed on immune cells like B cells, macrophages, and neutrophils. Phosphorylation at Tyr292 specifically occurs in the inhibitory FcγRIIB isoform, which contains an immunoreceptor tyrosine-based inhibitory motif (ITIM). Upon co-ligation with activating receptors (e.g., B-cell receptor), FcγRIIB becomes phosphorylated at Tyr292. recruiting phosphatases like SHIP to dampen cellular activation and maintain immune homeostasis. This phosphorylation event is critical for balancing immune responses and preventing hyperactivation. The CD32 (Phospho-Tyr292) antibody enables researchers to study this regulatory mechanism in contexts such as autoimmune diseases, cancer immunotherapy, or infectious immunity. It is widely used in techniques like Western blotting, immunofluorescence, and flow cytometry to assess FcγRIIB activation status, offering insights into therapeutic strategies targeting inhibitory signaling pathways. Proper controls (e.g., phosphorylation inhibitors) are essential to validate specificity.