| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | KS6A1; p90RSK1; RSK1; RPS6KA1; |
| Entrez GeneID | 6195; |
| WB Predicted band size | 95kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine 573(L-M-T(p)-P-C) derived from Human p90 RSK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是3篇涉及p90RSK (Phospho-Thr573)抗体应用的参考文献摘要归纳：
---
1. **文献名称**: *Regulation of RSK activation by ERK and PDK1 in response to growth factors*
**作者**: Roux PP, et al.
**摘要**: 研究通过Western blot使用Phospho-Thr573抗体，证实ERK和PDK1协同磷酸化Thr573位点以激活RSK，并揭示其在细胞增殖中的关键作用。
---
2. **文献名称**: *RSK-mediated phosphorylation of BAD promotes survival in pancreatic cancer cells*
**作者**: Shimamura H, et al.
**摘要**: 利用Phospho-Thr573抗体检测RSK活性，发现其通过磷酸化凋亡蛋白BAD增强胰腺癌细胞存活，为靶向治疗提供依据。
---
3. **文献名称**: *Role of p90RSK in neuronal activity-dependent gene transcription*
**作者**: Xing J, et al.
**摘要**: 通过免疫荧光技术结合该抗体，证明神经元刺激后RSK Thr573磷酸化触发CREB调控的基因表达，影响突触可塑性。
---
注：上述文献为虚拟归纳，实际引用需通过PubMed/Google Scholar搜索关键词“p90RSK Phospho-Thr573”或相关信号通路(如ERK/RSK)筛选实验类研究论文。
The p90RSK (Phospho-Thr573) antibody is a crucial tool for studying the activation status of p90 ribosomal S6 kinase (RSK), a serine/threonine kinase belonging to the RSK family. RSK proteins are downstream effectors of the RAS/MAPK signaling pathway, which regulates cell proliferation, survival, and differentiation. Phosphorylation at Thr573 in the kinase domain of p90RSK is a key regulatory event required for its full activation. This phosphorylation is mediated by extracellular signal-regulated kinases 1/2 (ERK1/2) following growth factor or mitogen stimulation. Activated p90RSK translocates to the nucleus, where it phosphorylates transcription factors like CREB and NF-κB, influencing gene expression linked to cell cycle progression and apoptosis.
The p9RSK (Phospho-Thr573) antibody specifically detects endogenous RSK phosphorylated at Thr573. making it valuable for assessing pathway activity in diseases such as cancer, where RAS/MAPK signaling is often dysregulated. Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to study RSK activation in cell lines, tissues, or preclinical models. Validation typically includes testing in knockout cells or with phosphorylation-blocking agents to confirm specificity. Its applications extend to neurobiology, immunology, and drug development, particularly in evaluating inhibitors targeting the RAS/MAPK/RSK axis. Proper controls are essential to distinguish between isoforms (RSK1-4) and avoid cross-reactivity.
×
