| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | APAF-3; MCH6; RNCASP9; CASP-9; ICE-LAP6 |
| Entrez GeneID | 842; |
| WB Predicted band size | 47kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of threonine 125 (P-E-T(p)-P-R) derived from Human Caspase 9. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Caspase 9 (Phospho-Thr125)抗体的3篇参考文献示例(文献为假设性概括，实际引用需核实)：
1. **文献名称**：*"ERK-mediated phosphorylation of Caspase-9 at Thr125 suppresses apoptosis"*
**作者**：Cardone M.H. et al.
**摘要**：该研究发现ERK激酶通过磷酸化Caspase-9的Thr125位点，抑制其蛋白酶活性，从而阻断凋亡信号通路。研究利用Phospho-Thr125特异性抗体验证了该修饰在细胞应激反应中的调控作用。
2. **文献名称**：*"Akt promotes cell survival by phosphorylating Caspase-9 at Thr125"*
**作者**：Fujita E. et al.
**摘要**：本文揭示Akt激酶介导的Caspase-9 Thr125磷酸化可阻止其与Apaf-1的结合，抑制凋亡体形成。通过Phospho-Thr125抗体进行免疫印迹分析，证实该修饰在肿瘤细胞抗凋亡机制中的关键地位。
3. **文献名称**：*"Threonine 125 phosphorylation of Caspase-9 regulates hypoxia-induced cardiomyocyte death"*
**作者**：Martin L.J. et al.
**摘要**：研究证明缺氧条件下Caspase-9 Thr125位点的磷酸化通过降低其酶活性保护心肌细胞免于凋亡。采用Phospho-Thr125抗体进行免疫荧光定位，发现该修饰与线粒体凋亡通路抑制相关。
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**注**：以上为模拟文献，实际研究需查阅具体数据库(如PubMed)。建议以“Caspase 9 Thr125 phosphorylation”为关键词检索，筛选涉及抗体应用的实验论文。
Caspase 9 is a key protease in the intrinsic apoptosis pathway, activated in response to cellular stress, DNA damage, or developmental signals. Its activation involves apoptosome formation, where procaspase-9 is recruited to Apaf-1 and undergoes conformational changes, leading to self-cleavage and generation of active subunits. Phosphorylation at specific residues, such as Thr125. plays a critical role in regulating Caspase-9 activity. Thr125 phosphorylation, mediated by kinases like ERK or CDK1. has been reported to suppress Caspase-9 activation by interfering with apoptosome assembly or substrate binding, thereby modulating apoptotic signaling. This post-translational modification highlights a mechanism of cellular survival regulation under stress conditions.
The Caspase 9 (Phospho-Thr125) antibody is a specialized tool designed to detect Caspase-9 phosphorylated at Thr125. It is widely used in research to study apoptosis regulation, kinase signaling crosstalk, and cellular responses to DNA damage or chemotherapeutic agents. By distinguishing phosphorylated Caspase-9 from its unmodified form, this antibody helps elucidate how post-translational modifications fine-tune apoptotic pathways. Validated in applications like Western blotting and immunoprecipitation, it serves as a critical reagent in cancer biology, neurobiology, and studies of diseases linked to apoptosis dysregulation. Understanding Caspase-9 phosphorylation dynamics may aid in developing therapeutic strategies targeting apoptosis-resistant cancers or degenerative disorders.
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