Rabbit Polyclonal CDC25A(Phospho-Tyr59) Antibody

The CDC25A (Phospho-Tyr59) antibody is a specialized tool used to detect the phosphorylated form of CDC25A at tyrosine residue 59. CDC25A, a dual-specificity phosphatase, plays a critical role in cell cycle regulation by dephosphorylating and activating cyclin-dependent kinases (CDKs), particularly during G1/S and G2/M transitions. Its activity is tightly controlled through post-translational modifications, including phosphorylation, which modulates stability, subcellular localization, and enzymatic function. Phosphorylation at Tyr59 is linked to regulatory mechanisms that may involve Src-family kinases or other tyrosine kinases, often in response to DNA damage or stress signals. This modification can promote CDC25A degradation via proteasomal pathways or inhibit its phosphatase activity, contributing to cell cycle arrest and repair processes. Dysregulation of CDC25A is associated with cancer progression, as its overexpression can drive uncontrolled proliferation. The CDC25A (Phospho-Tyr59) antibody is essential for studying phosphorylation-dependent CDC25A dynamics in signaling pathways, DNA damage responses, and oncogenesis. Researchers employ it in techniques like Western blotting, immunoprecipitation, and immunofluorescence to assess phosphorylation status, correlate it with functional outcomes, and explore therapeutic targets in diseases marked by cell cycle aberrations. Specific validation ensures minimal cross-reactivity with related phosphatases (e.g., CDC25B/C) or non-phosphorylated forms.