Rabbit Polyclonal FBXL3(Phospho-Ser320) Antibody

The FBXL3 (Phospho-Ser320) antibody is a specialized tool used to study the phosphorylation state of FBXL3 (F-box and leucine-rich repeat protein 3), a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex. FBXL3 plays a critical role in regulating circadian rhythms by targeting core clock proteins, such as CRY1 and CRY2. for ubiquitination and subsequent proteasomal degradation. Phosphorylation at Ser320 is a key post-translational modification that modulates FBXL3's stability, activity, or interactions with other proteins. This site is believed to influence FBXL3’s ability to bind CRY proteins, thereby affecting circadian clock dynamics. The FBXL3 (Phospho-Ser320) antibody is commonly employed in techniques like Western blotting, immunofluorescence, or immunoprecipitation to investigate phosphorylation-dependent regulatory mechanisms in circadian biology or related pathways. Its specificity for the phosphorylated form allows researchers to study how cellular signals, such as kinase activation or metabolic changes, regulate FBXL3 function. This antibody has become essential in exploring connections between circadian disruption, metabolic disorders, and cancer, where FBXL3-mediated protein turnover is implicated. Validation often includes testing in knockout models or phosphatase-treated samples to confirm target specificity.