Rabbit Polyclonal Bub3(Phospho-Tyr207) Antibody

The Bub3 (Phospho-Tyr207) antibody is designed to detect Bub3 protein phosphorylated at tyrosine residue 207. a post-translational modification implicated in regulating the spindle assembly checkpoint (SAC). Bub3 is a core component of the SAC, a surveillance mechanism ensuring accurate chromosome segregation during mitosis by delaying anaphase until all kinetochores are properly attached to spindle microtubules. It forms complexes with other checkpoint proteins, such as Bub1 and BubR1. to monitor kinetochore-microtubule interactions and generate the "wait anaphase" signal. Phosphorylation at Tyr207 is thought to modulate Bub3's interaction with binding partners or its localization during checkpoint activation. Studies suggest this modification may influence SAC signaling efficiency, although its precise mechanistic role remains under investigation. The Bub3 (Phospho-Tyr207) antibody serves as a tool to study SAC dynamics, mitotic errors, and genomic instability linked to diseases like cancer. Researchers utilize it in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess Bub3 activation states under conditions affecting mitotic fidelity. Specificity validation often includes phosphatase treatment or comparison with non-phosphorylated mutants. Dysregulation of Bub3 or its phosphorylation status has been associated with aneuploidy and tumor progression, highlighting its relevance in oncology research.