Rabbit Polyclonal Cdc25B(Phospho-Ser149) Antibody

The Cdc25B (Phospho-Ser149) antibody is a specialized tool for studying post-translational regulation of the Cdc25B phosphatase, a key cell cycle regulator. Cdc25B activates cyclin-dependent kinase 1 (CDK1)/Cyclin B complexes by removing inhibitory phosphorylation marks, driving G2/M phase transition. Phosphorylation at Ser149 represents a critical regulatory mechanism, often induced in response to DNA damage or replication stress. This modification is mediated by kinases such as Chk1 or Plk1. depending on cellular context, and can alter Cdc25B’s subcellular localization or stability. For instance, DNA damage triggers Ser149 phosphorylation, promoting cytoplasmic retention of Cdc25B to prevent premature mitotic entry, thereby maintaining cell cycle arrest for repair. The Cdc25B (Phospho-Ser149) antibody specifically detects this phosphorylation event, enabling researchers to study checkpoint activation, DNA damage response pathways, and cell cycle dysregulation in diseases like cancer. It is widely used in techniques including immunoblotting, immunofluorescence, and flow cytometry. Validation typically involves knockout/knockdown controls or peptide competition assays to ensure specificity. Dysregulated Cdc25B phosphorylation is implicated in tumorigenesis, making this antibody valuable for both basic research and preclinical studies targeting cell cycle checkpoints.