Rabbit Polyclonal syk(phospho-Tyr323) Antibody

中文名： syk(phospho-Tyr323)抗体
别名： Spleen tyrosine kinase

货号: IPDX40329

Price： ￥1280

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	咨询技术	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	1/50-1/100	Human,Mouse,Rat
ICC	1/100-1/200	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Spleen tyrosine kinase
Entrez GeneID	6850;
WB Predicted band size	72kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human,Mouse,Rat
Immunogen	Peptide sequence around phosphorylation site of tyrosine 323 (N-P-Y(p)-E- P) derived from Human syk.
Formulation	Purified antibody in PBS with 0.05% sodium azide.

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Western blot analysis of extracts from JK cells untreated or treated with H2O2 for the indicated times, using syk(phospho-Tyr323) Antibody P40329.
Immunofluorescence staining of methanol-fixed Hela cells using syk(phospho-Tyr323) Antibody P40329.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using syk(Phospho-Tyr323) Antibody P40329(left) or the same antibody preincubated with blocking peptide(right).

参考文献

以下是关于SYK(phospho-Tyr323)抗体的参考文献示例(注：文献为虚构示例，实际需通过数据库验证)：

1. **文献名称**：*"SYK phosphorylation at Tyr323 is critical for B-cell receptor signaling"*

**作者**：Smith A et al.

**摘要**：研究揭示了SYK在Tyr323位点的磷酸化对B细胞受体信号传导的调控作用，通过Western blot和免疫沉淀验证了该抗体在检测SYK激活状态中的特异性。

2. **文献名称**：*"Role of SYK-Y323 phosphorylation in mast cell degranulation"*

**作者**：Chen L et al.

**摘要**：探讨SYK-Tyr323磷酸化在肥大细胞脱颗粒中的作用，利用该抗体证明抗原刺激后SYK活性与炎症反应的相关性。

3. **文献名称**：*"Phospho-SYK (Y323) as a biomarker in chronic lymphocytic leukemia"*

**作者**：Rodriguez B et al.

**摘要**：通过流式细胞术和免疫组化分析，证实SYK-Tyr323磷酸化水平与白血病细胞存活和耐药性相关，提示其临床诊断价值。

4. **文献名称**：*"Structural basis of SYK activation by tyrosine phosphorylation"*

**作者**：Kimura T et al.

**摘要**：结合晶体学与生化实验，阐明Tyr323磷酸化对SYK激酶构象的影响，抗体用于验证体外磷酸化实验结果。

**建议**：通过PubMed或Google Scholar搜索关键词“SYK Y323 phosphorylation antibody”或相关术语，筛选实验方法学或疾病机制研究的具体文献。

背景信息

The Syk (phospho-Tyr323) antibody is a specialized tool for studying the activation and regulation of spleen tyrosine kinase (Syk), a non-receptor tyrosine kinase critical in immune receptor signaling. Syk plays a central role in signaling pathways downstream of B-cell receptors (BCR), Fc receptors, and integrins, mediating cellular responses such as proliferation, differentiation, and phagocytosis. Phosphorylation at Tyr323 (human) or Tyr342 (mouse) is a key regulatory event that modulates Syk activity. This autophosphorylation site lies within the linker region between the two SH2 domains, and its phosphorylation is associated with enhanced kinase activity, conformational changes, and recruitment of downstream signaling molecules.

The Syk (phospho-Tyr323) antibody specifically detects Syk when phosphorylated at this residue, enabling researchers to study its activation status in various contexts. It is widely used in immunoblotting (WB), immunohistochemistry (IHC), and flow cytometry to investigate Syk-driven signaling in immune cells, particularly in B cells, mast cells, and macrophages. Dysregulated Syk activity is implicated in autoimmune diseases (e.g., rheumatoid arthritis), allergies, and hematologic malignancies, making this antibody valuable for both basic research and translational studies targeting Syk-related pathologies. Validation typically includes testing in knockout models or phosphorylation-blocking assays to ensure specificity.