| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Map2k5 |
| Entrez GeneID | 29568; |
| WB Predicted band size | 46kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 311 and threonine 315 (V-N-S(p)I-A-K-T(p)-Y-V) derived from Rat MEK5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是基于MEK5磷酸化位点(Ser311/Thr315)相关抗体的模拟参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**：*MEK5 Phosphorylation at Ser311/Thr315 Mediates ERK5 Activation in Hypoxia-Induced Angiogenesis*
**作者**：Li, X. et al.
**摘要**：研究揭示低氧条件下MEK5在Ser311/Thr315位点的磷酸化通过激活下游ERK5通路促进血管生成，使用特异性抗体验证了该磷酸化事件在肿瘤微环境中的作用。
2. **文献名称**：*Development of a Phospho-Specific Antibody for MEK5 (Ser311/Thr315) and Its Application in Cardiac Hypertrophy Models*
**作者**：Wang, Y. et al.
**摘要**：本文报道了一种针对MEK5双磷酸化位点(Ser311/Thr315)抗体的开发与验证，并通过心肌细胞肥大模型证明该抗体在检测压力超负荷诱导的MEK5激活中的特异性。
3. **文献名称**：*MEK5/ERK5 Signaling in Inflammatory Response: Role of Phosphorylation at Ser311/Thr315*
**作者**：Smith, J. et al.
**摘要**：利用MEK5(p-Ser311/Thr315)抗体，研究发现LPS刺激的巨噬细胞中MEK5磷酸化水平升高，表明其在炎症信号传导中调控ERK5的核转位及促炎基因表达。
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**备注**：以上为模拟示例，真实文献需通过PubMed/Google Scholar以关键词“MEK5 phospho-Ser311”、“MEK5 Thr315 antibody”等检索。建议结合具体研究场景筛选近期论文。
The MEK5 (phospho-Ser311/Thr315) antibody is a specialized tool used to detect the activated form of MAPK/ERK kinase 5 (MEK5), a key component of the mitogen-activated protein kinase (MAPK) signaling pathway. MEK5 regulates downstream extracellular signal-regulated kinase 5 (ERK5), which mediates cellular responses to growth factors, stress, and inflammatory signals. Phosphorylation at Ser311 and Thr311 (or Thr315. depending on isoform numbering) is critical for MEK5 activation, triggering conformational changes that enhance its kinase activity and promote ERK5 phosphorylation. This dual phosphorylation event facilitates MEK5's role in processes like proliferation, differentiation, and survival, particularly in contexts such as cardiovascular development, cancer progression, and neuronal signaling.
The antibody specifically recognizes MEK5 when phosphorylated at these residues, enabling researchers to study activation dynamics in cell or tissue lysates via techniques like Western blotting, immunofluorescence, or immunoprecipitation. Its applications include investigating MEK5/ERK5 pathway regulation under stimuli (e.g., oxidative stress, growth factors) or in disease models (e.g., tumors, cardiovascular disorders). Validation typically involves testing specificity using phosphorylation-deficient mutants or phosphatase-treated samples. Reliable detection of phospho-MEK5 aids in understanding pathway crosstalk, drug targeting, and MEK5's dual role as both a kinase and a nuclear transcriptional regulator when complexed with ERK5.
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