| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CMH10, MFM12, MLC-2, MLC-2s/v, MLC-2v, MLC2 |
| Entrez GeneID | 4633; |
| WB Predicted band size | 20kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of serine 15 (A-N-S(p)-N-V) derived from Human MLC2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与MLC2 (Phospho-Ser15)抗体相关的参考文献示例(注：文献为模拟虚构，供参考)：
1. **"Phosphorylation of Myosin Light Chain 2 at Ser15 Regulates Cell Migration in Tumor Cells"**
- **作者**: Chen et al., Journal of Cell Biology
- **摘要**: 本研究通过MLC2 (Phospho-Ser15)抗体的特异性检测，发现肿瘤细胞迁移过程中Ser15位点的磷酸化水平显著升高，且该修饰通过激活肌球蛋白收缩性促进细胞伪足形成。
2. **"Role of MLC2 Phosphorylation in Smooth Muscle Contraction"**
- **作者**: Smith et al., Journal of Biological Chemistry
- **摘要**: 利用MLC2 (Phospho-Ser15)抗体进行免疫印迹分析，证实MLCK介导的Ser15磷酸化是平滑肌收缩的关键步骤，并揭示其与钙离子信号通路的关联。
3. **"Dynamic Phosphorylation of MLC2 Ser15 During Mitosis"**
- **作者**: Tanaka et al., Molecular Biology of the Cell
- **摘要**: 通过免疫荧光技术结合MLC2 (Phospho-Ser15)抗体，发现该位点的磷酸化在细胞分裂末期调控收缩环的形成，影响胞质分裂的完成。
4. **"Validation of a Phospho-Specific Antibody for MLC2 Ser15 in Cardiac Muscle Models"**
- **作者**: Lee et al., Circulation Research
- **摘要**: 本研究验证了MLC2 (Phospho-Ser15)抗体的特异性，并在心肌细胞模型中证明Ser15磷酸化水平与心脏收缩功能障碍的相关性。
(提示：实际文献需通过PubMed/Google Scholar以关键词“MLC2 Phospho-Ser15 antibody”或“MYL2 phospho-Ser15”检索获取。)
The MLC2 (Phospho-Ser15) antibody is a crucial tool for studying the phosphorylation status of myosin light chain 2 (MLC2) at serine 15. a post-translational modification linked to cellular contractility and motility. MLC2 is a regulatory component of myosin II, a motor protein essential for muscle contraction and non-muscle cellular processes like cytokinesis, migration, and maintenance of cell shape. Phosphorylation at Ser15. mediated by kinases such as myosin light-chain kinase (MLCK) or Rho-associated kinase (ROCK), promotes actin-myosin interaction by inducing conformational changes in myosin II. This activates contractile forces in smooth muscle cells, endothelial cells, and migrating cells.
Researchers use the MLC2 (Phospho-Ser15) antibody to investigate pathways regulating vascular tone, wound healing, cancer cell invasion, and immune cell trafficking. It is widely applied in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect phosphorylation dynamics in response to stimuli (e.g., calcium signaling, Rho/ROCK activation) or pathological conditions (e.g., hypertension, metastasis). Specificity validation via knockout controls or phosphatase treatment is recommended to ensure accurate detection. The antibody’s utility spans basic research in cell biology and translational studies targeting diseases involving dysregulated contractility, such as cardiovascular disorders or tumor progression.
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