| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Fumarase; fumarate hydratase; Fumarate hydratase, mitochondrial; FUMH; HLRCC;聽 |
| Entrez GeneID | 2271; |
| WB Predicted band size | 54kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 236(T-H-T(p)-Q-D) derived from Human FH. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是模拟生成的关于FH(Phospho-Thr236)抗体的参考文献示例(非真实文献，仅供格式参考)：
---
1. **"Phosphorylation of Fumarate Hydratase at Thr236 Modulates Its Enzymatic Activity in Renal Cancer"**
*Author: Smith A, et al.*
摘要：研究揭示了FH在Thr236位点的磷酸化通过变构调节其延胡索酸酶活性，并促进HIF-1α信号通路的激活，为HLRCC相关肾癌的代谢异常提供了机制解释。该文献详细验证了Phospho-Thr236抗体的特异性及在肿瘤组织中的检测应用。
2. **"A Novel Phospho-Specific Antibody Reveals Dynamic FH (Thr236) Phosphorylation During Cell Cycle Progression"**
*Author: Chen L, et al.*
摘要：开发了一种高特异性Phospho-Thr236抗体，发现FH在G1/S期转换时Thr236磷酸化水平升高，提示其可能通过调控细胞周期相关代谢酶活性影响肿瘤增殖。
3. **"Targeting FH Phosphorylation as a Therapeutic Strategy in Hereditary Leiomyomatosis"**
*Author: Rodriguez M, et al.*
摘要：利用Phospho-Thr236抗体分析了HLRCC患者样本中FH磷酸化状态，发现磷酸化水平与疾病严重程度相关，并筛选出小分子抑制剂可靶向该修饰位点，为治疗提供新思路。
4. **"Validation of Anti-Phospho-Thr236 FH Antibody in Metabolic Stress Models"**
*Author: Kim J, et al.*
摘要：系统验证了该抗体在缺氧和营养缺乏条件下的适用性，证实Thr236磷酸化响应氧化应激并调控线粒体功能，强调了其在代谢研究中的工具价值。
---
**注**：以上文献为模拟生成，实际研究中请通过PubMed/Google Scholar等平台检索真实文献(关键词如"Fumarate Hydratase phosphorylation"+"antibody validation")。若需真实文献协助，请提供更具体的研究背景。
The FH (Phospho-Thr236) antibody is a specialized tool used to detect fumarate hydratase (FH) when phosphorylated at threonine residue 236. FH is a critical enzyme in the tricarboxylic acid (TCA) cycle, catalyzing the reversible hydration/dehydration of fumarate to malate. Post-translational modifications, such as phosphorylation, regulate FH's enzymatic activity, subcellular localization, or interactions with other proteins. The Thr236 phosphorylation site is implicated in modulating FH function under specific cellular conditions, such as metabolic stress or signaling pathway activation.
This antibody is particularly valuable in studying FH's role in cellular metabolism, cancer biology, and mitochondrial dysfunction. Dysregulation of FH is linked to hereditary leiomyomatosis and renal cell cancer (HLRCC), where FH mutations cause accumulation of fumarate, driving oncogenic signaling. Phosphorylation at Thr236 may influence FH’s metabolic activity or non-canonical roles in DNA damage response or hypoxia signaling.
Researchers employ the FH (Phospho-Thr236) antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess phosphorylation status in cell lines, tissues, or disease models. Its specificity helps elucidate how FH phosphorylation contributes to metabolic adaptation, redox balance, or tumor progression, offering insights into therapeutic targeting of FH-related pathways.
×
