| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CAP4; ICE8; MACH; MCH5; Mch-5 |
| Entrez GeneID | 1050; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 21 (L-Q-S(p)-P-P) derived from Human C/EBP-α. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于C/EBP-α (Phospho-Ser21)抗体的代表性文献摘要(模拟示例，非真实文献)：
1. **文献名称**：*Phosphorylation of C/EBPα at Ser21 regulates adipogenesis through HDAC1 interaction*
**作者**：Wang et al. (2015)
**摘要**：研究证明C/EBP-α在Ser21位点的磷酸化通过招募组蛋白去乙酰化酶HDAC1抑制脂肪细胞分化，使用Phospho-Ser21抗体验证了该位点在分化过程中的动态修饰。
2. **文献名称**：*Ser21 phosphorylation modulates C/EBPα transcriptional activity in acute myeloid leukemia*
**作者**：Smith & Jones (2018)
**摘要**：通过Phospho-Ser21抗体检测发现，白血病细胞中C/EBP-α的Ser21磷酸化水平异常升高，导致其促分化功能受阻，与疾病进展相关。
3. **文献名称**：*MAPK-mediated C/EBPα phosphorylation at Ser21 promotes hepatic gluconeogenesis*
**作者**：Chen et al. (2020)
**摘要**：利用Phospho-Ser21特异性抗体证实，肝细胞中MAPK通路通过磷酸化C/EBP-α Ser21增强其与PGC-1α的结合，促进糖异生关键基因表达。
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**注**：以上为模拟文献，实际文献需通过PubMed或抗体供应商官网(如CST、Abcam)查询具体抗体货号对应的引用文献。
The C/EBP-α (CCAAT/enhancer-binding protein alpha) is a transcription factor critical for cellular differentiation, metabolism regulation, and growth arrest in various tissues, including liver, adipose, and hematopoietic systems. Phosphorylation at Ser21 (Ser21 in human; corresponds to Ser20 in mouse) is a key post-translational modification modulating its activity. This phosphorylation, often mediated by signaling pathways like MAPK/ERK, attenuates C/EBP-α’s DNA-binding ability and transcriptional activity, influencing processes such as adipogenesis, gluconeogenesis, and hematopoiesis.
The C/EBP-α (Phospho-Ser21) antibody is a specialized tool designed to detect the phosphorylated form of C/EBP-α at Ser21. It is widely used in research to study dynamic regulatory mechanisms in cellular differentiation, metabolic disorders, and cancer. For example, aberrant phosphorylation at this site has been linked to impaired differentiation in acute myeloid leukemia (AML) and insulin resistance in metabolic syndromes.
Validated in techniques like Western blotting, immunoprecipitation, and immunofluorescence, this antibody helps elucidate context-dependent roles of C/EBP-α phosphorylation. Researchers utilize it to explore how kinase/phosphatase pathways modulate C/EBP-α’s functional switch between pro-differentiation and proliferation-suppressive roles, offering insights into therapeutic targeting for diseases involving C/EBP-α dysregulation.
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