| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LAG; LAP18; STN1; Prosolin; stathmin |
| Entrez GeneID | 6857; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 202(R-K-T(p)-L-N) derived from Human Synaptotagmin. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下为3篇涉及Synaptotagmin磷酸化(Thr202位点)及其抗体的参考文献摘要：
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1. **标题**: *Synaptotagmin-1 phosphorylation regulates calcium-dependent exocytosis*
**作者**: Lee HK et al.
**摘要**: 研究报道Syt1 Thr202位点的磷酸化通过Ca²⁺依赖性激酶调控突触囊泡与质膜的融合效率，抗体用于检测神经元活动依赖性磷酸化修饰。
2. **标题**: *Phosphorylation of Synaptotagmin at Thr202 modulates synaptic short-term plasticity*
**作者**: Cohen R et al.
**摘要**: 通过Phospho-Thr202抗体发现，Syt1该位点的磷酸化影响突触囊泡的释放动力学，从而调控短期可塑性和神经递质释放概率。
3. **标题**: *Activity-dependent phosphorylation of Synaptotagmin-1 in hippocampal neurons*
**作者**: Xu J et al.
**摘要**: 利用Phospho-Thr202抗体证实，海马神经元中Syt1的Thr202磷酸化水平与突触活动增强相关，提示其在突触功能调控中的作用。
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注：上述文献为示例性质，实际引用需根据具体研究通过PubMed或Google Scholar检索关键词如“Synaptotagmin Phospho-Thr202 antibody”获取。部分抗体厂商(如Cell Signaling Technology)也可能提供相关应用文献。
Synaptotagmin (Syt) is a calcium-binding protein family critical for synaptic vesicle exocytosis and neurotransmitter release. Among its isoforms, synaptotagmin-1 (Syt1) serves as a primary calcium sensor in presynaptic terminals, regulating the fusion of synaptic vesicles with the plasma membrane. The phosphorylation of Syt1 at specific residues, such as threonine 202 (Thr202), modulates its interaction with SNARE complexes and other regulatory proteins, influencing synaptic transmission efficiency and plasticity. Phosphorylation at Thr202 is thought to be mediated by kinases like protein kinase C (PKC) or calcium/calmodulin-dependent kinase II (CaMKII), often in response to neuronal activity or signaling cascades.
The Synaptotagmin (Phospho-Thr202) antibody is a selective tool designed to detect Syt1 phosphorylated at Thr202. It enables researchers to study activity-dependent post-translational modifications of Syt1 in contexts such as synaptic plasticity, learning, and memory. This antibody is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to map phosphorylation dynamics in neuronal tissues or cultured cells. Its applications extend to investigating pathological conditions, including neurodegenerative diseases (e.g., Alzheimer’s) and epilepsy, where dysregulated synaptic protein phosphorylation is implicated. Validation typically involves knockout controls or phosphatase treatment to confirm specificity. Cross-reactivity with non-target phospho-proteins should be assessed for accurate interpretation.
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