| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AGMX1; ATK; kinase Btk; BPK; |
| Entrez GeneID | 695; |
| WB Predicted band size | 80kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 223 (A-L-Y(p)-D-Y) derived from Human BTK. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BTK (Phospho-Tyr223)抗体的3篇参考文献示例(内容为示例，非真实文献)：
1. **"Bruton's tyrosine kinase activation through phosphorylation at Tyr223 mediates B-cell receptor signaling"**
- **作者**: Smith A et al.
- **摘要**: 本研究揭示了BTK在Tyr223位点的磷酸化是其激活的关键步骤，通过特异性抗体检测验证了该磷酸化事件在B细胞受体信号转导中的功能，并证明其与下游靶蛋白的相互作用。
2. **"Phosphorylation dynamics of Bruton's tyrosine kinase in lymphoma progression"**
- **作者**: Johnson R et al.
- **摘要**: 利用Phospho-Tyr223特异性抗体，作者分析了BTK在B细胞淋巴瘤中的激活状态，发现Tyr223磷酸化水平与疾病进展和药物耐药性显著相关，为靶向治疗提供依据。
3. **"Development of a monoclonal antibody targeting phosphorylated Tyr223 on BTK for autoimmune disease research"**
- **作者**: Lee H et al.
- **摘要**: 本文报道了一种高特异性抗体的开发，用于检测BTK Tyr223磷酸化位点，并通过体外实验证明该抗体在类风湿性关节炎模型中对BTK活性监测的有效性。
(注：以上文献为模拟示例，实际引用需查询真实数据库如PubMed。)
The Bruton's tyrosine kinase (BTK), a member of the Tec kinase family, is a critical regulator of B-cell receptor (BCR) signaling. Its activation involves sequential phosphorylation events: first at Tyr551 in the kinase domain, followed by autophosphorylation at Tyr223 in the SH3 domain. Phosphorylation at Tyr223 (pY223) stabilizes BTK’s active conformation and modulates interactions with downstream signaling proteins, influencing B-cell development, differentiation, and survival. Dysregulated BTK activity is linked to X-linked agammaglobulinemia (XLA) and B-cell malignancies like chronic lymphocytic leukemia (CLL).
The BTK (Phospho-Tyr223) antibody specifically detects BTK phosphorylated at Tyr223. serving as a key tool to study BTK activation status in signaling pathways. Researchers use this phosphospecific antibody in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate BTK’s role in disease mechanisms or therapeutic responses. For instance, it helps evaluate the efficacy of BTK inhibitors (e.g., ibrutinib) in blocking BCR signaling, a therapeutic strategy for B-cell cancers. By distinguishing activated BTK from its inactive form, this antibody provides insights into pathway dynamics, drug resistance, and molecular targeting in both basic research and clinical contexts. Its specificity ensures accurate detection of phosphorylation-dependent BTK interactions, aiding the development of targeted therapies.
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