| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | ARR1; arrestin 2; arrestin beta 1; beta-arrestin-1; |
| Entrez GeneID | 408; |
| WB Predicted band size | 50kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 412 (T-G-S(p)-P-Q) derived from Human Arrestin 1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Arrestin 1 (Phospho-Ser412)** 抗体的示例参考文献(仅供参考，实际文献需通过数据库验证)：
1. **文献名称**：*"Phosphorylation of Arrestin1 at Ser412 regulates its interaction with rhodopsin in retinal photoreceptors"*
**作者**：Gurevich VV, et al.
**摘要**：研究揭示了Arrestin1在Ser412位点的磷酸化如何调控其与视紫红质(rhodopsin)的结合能力，通过免疫印迹和免疫荧光技术证实该磷酸化修饰影响光信号转导的终止效率。
2. **文献名称**：*"Dynamic phosphorylation of arrestin1 controls light-dependent subcellular localization in rod cells"*
**作者**：Chen J, et al.
**摘要**：利用Phospho-Ser412特异性抗体，发现光照条件下Arrestin1在Ser412位点的磷酸化促进其从胞质向光感受器外段的转位，进而参与视觉适应过程。
3. **文献名称**：*"Role of β-Arrestin1 phosphorylation in GPCR internalization: Insights from Ser412 mutagenesis"*
**作者**：Luttrell LM, et al.
**摘要**：通过构建Ser412突变体并结合Phospho-Ser412抗体检测，证明该位点的磷酸化是β-Arrestin1介导GPCR内吞的关键调控步骤。
4. **文献名称**：*"Disease-associated mutations in Arrestin1 alter phosphorylation-dependent protein stability"*
**作者**：Smith SJ, et al.
**摘要**：研究发现遗传性视网膜病变患者的Arrestin1 Ser412磷酸化水平异常，该抗体被用于评估磷酸化状态与蛋白稳定性及疾病表型的关联。
**提示**：以上为模拟示例，建议通过PubMed或Google Scholar搜索关键词“Arrestin1 Ser412 phosphorylation antibody”或相关研究团队(如Gurevich, Luttrell)的论文获取真实文献。
Arrestin 1 (also known as S-antigen or SAG) is a cytosolic protein belonging to the arrestin family, primarily involved in regulating G protein-coupled receptor (GPCR) signaling. It plays a critical role in desensitizing activated receptors, such as rhodopsin in the retina, by binding to phosphorylated GPCRs and blocking further G protein activation. Phosphorylation of Arrestin 1 at Serine 412 (Ser412) is a key post-translational modification that modulates its conformation, stability, and interaction with receptors. This phosphorylation event enhances its ability to translocate to the membrane and engage with activated receptors, thereby fine-tuning cellular responses to extracellular stimuli.
The Arrestin 1 (Phospho-Ser412) antibody is a specialized tool designed to detect the phosphorylated form of Arrestin 1 at Ser412. It is widely used in research to study the activation and regulatory mechanisms of Arrestin 1 in GPCR signaling pathways, particularly in retinal photoreceptor cells and other tissues where Arrestin 1 is expressed. Applications include Western blotting, immunohistochemistry, and immunofluorescence to assess phosphorylation-dependent changes in subcellular localization or protein interactions. Validated in specific experimental models, this antibody helps elucidate the role of Arrestin 1 in diseases linked to dysregulated GPCR signaling, such as retinal degeneration, cancer, and neurological disorders. Proper controls, including non-phosphorylated antigen blocking, are recommended to ensure specificity.
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