| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | C-C CKR-5; CKR5; CHEMR13; CMKBR5; CCR-5 |
| Entrez GeneID | 1234; |
| WB Predicted band size | 40kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 349 (E-I-S(p)-V-G) derived from Human CCR5. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于CCR5 (Phospho-Ser349)抗体的参考文献示例(注：以下内容为示例性概括，具体文献可能需要通过数据库进一步验证)：
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1. **Title**: "Phosphorylation of CCR5 at Ser349 promotes HIV-1 entry and signaling"
**Author**: Smith A, et al.
**Summary**: 该研究通过Western blot和免疫荧光技术，使用Phospho-Ser349特异性抗体，揭示了CCR5在Ser349位点的磷酸化对HIV-1病毒进入宿主细胞的关键作用，并表明该修饰增强了受体的构象变化和信号转导。
2. **Title**: "Regulation of chemokine receptor CCR5 by post-translational modifications"
**Author**: Lee J, et al.
**Summary**: 作者利用Phospho-Ser349抗体，发现炎症刺激下CCR5的Ser349磷酸化水平显著升高，并通过功能实验证明该修饰介导了受体内吞和下游MAPK信号通路的激活，为靶向CCR5的免疫治疗提供新思路。
3. **Title**: "GRK-mediated phosphorylation of CCR5 modulates T cell migration"
**Author**: Chen X, et al.
**Summary**: 研究通过免疫沉淀和Phospho-Ser349抗体验证了G蛋白偶联受体激酶(GRK)对CCR5的Ser349位点的磷酸化调控，表明这一修饰抑制了T细胞的趋化性迁移，可能在自身免疫疾病中发挥调控作用。
4. **Title**: "Development and validation of a phosphospecific antibody for CCR5 Ser349"
**Author**: Rodriguez B, et al.
**Summary**: 该文献详细描述了Phospho-Ser349抗体的开发过程，包括表位设计、特异性验证(如肽段竞争实验)，并应用于检测癌症模型中CCR5的磷酸化状态，证实其与肿瘤转移的相关性。
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**建议**：以上为基于领域知识的示例，实际文献建议通过PubMed或Google Scholar搜索关键词“CCR5 Phospho-Ser349 antibody”或“CCR5 Ser349 phosphorylation”获取最新研究。
The CCR5 (Phospho-Ser349) antibody is a specialized tool used to detect the phosphorylated form of the C-C chemokine receptor type 5 (CCR5) at serine residue 349. CCR5. a G protein-coupled receptor (GPCR), plays a critical role in immune cell migration and inflammation by binding chemokines like CCL3. CCL4. and CCL5. Phosphorylation of Ser349 is a key post-translational modification linked to receptor desensitization, internalization, and downstream signaling regulation. This site-specific phosphorylation is often triggered by ligand binding or cellular activation, facilitating interactions with β-arrestin to terminate signaling or redirect pathways, such as MAPK or PI3K/Akt cascades.
In research, the CCR5 (Phospho-Ser349) antibody is essential for studying receptor activation dynamics in diseases like HIV infection, where CCR5 serves as a co-receptor for viral entry. Phosphorylation status may influence viral tropism or therapeutic responses to CCR5 antagonists (e.g., maraviroc). It also aids in exploring cancer metastasis, autoimmune disorders, and inflammatory conditions where CCR5 signaling modulates immune cell recruitment. By enabling precise detection of phosphorylated CCR5 in techniques like Western blotting, immunofluorescence, or flow cytometry, this antibody supports mechanistic insights into receptor regulation and drug development targeting GPCR signaling networks. Its specificity ensures minimal cross-reactivity with non-phosphorylated CCR5 or related receptors like CCR2 or CXCR4.
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