| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | JAK2; Janus kinase 2; kinase Jak2; |
| Entrez GeneID | 3717; |
| WB Predicted band size | 125kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 570(G-D-Y(p)-G-Q) derived from Human JAK2 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于JAK2 (Phospho-Tyr570)抗体的3篇参考文献，按文献内容简要总结：
1. **文献名称**: *"JAK2 autoactivation and cytokine signaling"*
**作者**: Saharinen, P. et al.
**摘要**: 研究JAK2自磷酸化机制，发现Tyr570磷酸化对激酶活性调控至关重要。该文献使用Phospho-Tyr570特异性抗体验证了JAK2在细胞因子刺激下的自磷酸化位点，并揭示其与信号传导活性的关联。
2. **文献名称**: *"Phosphorylation of JAK2 at Tyr570 regulates hematopoietic stem cell function"*
**作者**: Li, J. et al.
**摘要**: 通过Phospho-Tyr570抗体的免疫印迹分析，发现JAK2-Tyr570磷酸化异常会破坏造血干细胞的自我更新能力，提示该位点磷酸化在血液系统疾病中的潜在病理作用。
3. **文献名称**: *"Structural insights into JAK2 activation by phosphorylation"*
**作者**: Bandaranayake, R.M. et al.
**摘要**: 结合晶体结构与细胞实验，利用Phospho-Tyr570抗体证实Tyr570磷酸化诱导JAK2构象变化，促进激酶结构域活化，为靶向该位点的药物设计提供理论依据。
**备注**：由于JAK2-Tyr570研究相对较少，部分文献可能侧重于机制而非抗体应用。若需更详细或更新的文献，建议通过PubMed或抗体供应商(如CST、Abcam)的产品引用列表进一步检索。
The JAK2 (Phospho-Tyr570) antibody is a specialized tool used to detect the phosphorylated form of tyrosine residue 570 (Y570) in Janus kinase 2 (JAK2), a critical non-receptor tyrosine kinase involved in cytokine signaling. JAK2 plays a central role in mediating signals from receptors for erythropoietin, thrombopoietin, and growth hormones, regulating processes like hematopoiesis, immune response, and cell proliferation. Its activation typically involves auto-/trans-phosphorylation at specific tyrosine residues, including Y570. which lies within the JH2 (pseudokinase) domain. Phosphorylation at Y570 is implicated in modulating JAK2's enzymatic activity by relieving autoinhibitory interactions between the JH1 (kinase) and JH2 domains, thereby promoting downstream signaling via the STAT pathway.
This antibody is widely employed in research to investigate JAK2 activation dynamics in physiological and pathological contexts, such as myeloproliferative neoplasms (MPNs), leukemia, and autoimmune disorders. Mutations in JAK2 (e.g., V617F) are linked to constitutive activation and disease, but phosphorylation at Y570 reflects a regulatory step in wild-type JAK2 signaling. Researchers use the antibody in techniques like Western blotting, immunofluorescence, or immunohistochemistry to assess JAK2 activation status, study signaling crosstalk, or evaluate therapeutic inhibitors targeting JAK2 activity. Its specificity for the phosphorylated Y570 epitope makes it valuable for dissecting JAK2-related mechanisms in both basic and translational studies.
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