Rabbit Polyclonal PEA-15(Phospho-Ser104) Antibody

The PEA-15 (Phospho-Ser104) antibody is designed to specifically detect the phosphorylation of serine residue 104 (Ser104) on the Phosphoprotein Enriched in Astrocytes 15 (PEA-15), a 15 kDa protein predominantly expressed in astrocytes but also found in other cell types. PEA-15 plays a dual role in regulating apoptosis and cell proliferation by interacting with key signaling molecules like extracellular signal-regulated kinase (ERK) and Fas-associated death domain (FADD) protein. Its activity is tightly modulated by phosphorylation at two serine residues, Ser104 and Ser116. which influence its subcellular localization and binding partners. Phosphorylation at Ser104. mediated by kinases such as protein kinase C (PKC) or calcium/calmodulin-dependent kinase II (CaMKII), enhances PEA-15's cytoplasmic retention, thereby inhibiting ERK-dependent transcriptional activity and promoting cell survival. This post-translational modification is implicated in pathological conditions, including cancer, diabetes, and neurological disorders, where dysregulated PEA-15 signaling contributes to disease progression. The PEA-15 (Phospho-Ser104) antibody serves as a critical tool for studying phosphorylation-dependent mechanisms in cellular models and tissues, enabling researchers to explore its role in apoptosis resistance, metabolic regulation, and therapeutic targeting. Validation of this antibody typically involves techniques like Western blotting, immunohistochemistry, and immunofluorescence to ensure specificity for the phosphorylated epitope.