| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC25; GNRP; GRF1; RASGRF1; |
| Entrez GeneID | 5923; |
| WB Predicted band size | 145kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of Serine 916(R-M-S(p)-L-A) derived from Mouse Ras-GRF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于 Ras-GRF1 (Phospho-Ser916) 抗体的3篇参考文献(基于公开文献的模拟示例，仅供参考)：
1. **文献名称**: *Calcium-dependent phosphorylation of Ras-GRF1 by NMDA receptor activation*
**作者**: Farnsworth CL, et al.
**摘要**: 研究揭示了NMDA受体激活后，钙离子信号通过CaMKII诱导Ras-GRF1的Ser916位点磷酸化，从而增强其鸟苷酸交换活性，促进Ras/MAPK通路信号传导。文中使用Phospho-Ser916抗体验证了该磷酸化事件。
2. **文献名称**: *Phosphorylation of Ras-GRF1 at Ser916 modulates synaptic plasticity and memory formation*
**作者**: Li Y, et al.
**摘要**: 通过Phospho-Ser916特异性抗体检测发现，小鼠海马神经元中Ras-GRF1的Ser916磷酸化水平与长时程增强(LTP)及空间记忆能力密切相关，提示该位点磷酸化在突触可塑性中的关键作用。
3. **文献名称**: *Ras-GRF1 mediates oxidative stress-induced neuronal apoptosis via phosphorylation at Ser916*
**作者**: Park J, et al.
**摘要**: 研究证明，氧化应激通过激活JNK激酶促使Ras-GRF1的Ser916磷酸化，进而激活下游Rac1通路诱导神经元凋亡。实验采用Phospho-Ser916抗体进行免疫印迹和免疫荧光分析。
**注**：以上文献为示例，实际引用需以真实发表的论文为准。建议通过PubMed或Google Scholar以关键词“Ras-GRF1 Ser916 phosphorylation”或“Ras-GRF1 Phospho-Ser916 antibody”检索最新研究。
The Ras-GRF1 (Phospho-Ser916) antibody is designed to detect the phosphorylation status of Ras-GRF1 at serine residue 916. a post-translational modification critical for regulating its activity. Ras-GRF1 (Ras-specific guanine nucleotide-releasing factor 1), also known as CDC25Mm, is a calcium/calmodulin-dependent guanine nucleotide exchange factor (GEF) that activates Ras and Rac GTPases, linking extracellular signals to intracellular pathways involved in cell growth, differentiation, and synaptic plasticity. Phosphorylation at Ser916. located within the autoinhibitory domain, is associated with conformational changes that relieve autoinhibition, enabling GEF activity and downstream signaling. This modification is often triggered by calcium influx or receptor tyrosine kinase activation.
The antibody is widely used in neuroscience and cancer research to study Ras-GRF1 activation mechanisms in contexts like synaptic transmission, memory formation, or oncogenesis. Its specificity for the phosphorylated form allows researchers to assess dynamic signaling events in response to stimuli such as neurotransmitters, growth factors, or stress conditions. Validation typically includes Western blotting, immunohistochemistry, or immunoprecipitation using cell lysates or tissue samples from brain, pancreas, or cancer models. Proper controls (e.g., phosphorylation-blocking mutants or phosphatase treatment) are essential to confirm signal specificity. Aberrant Ras-GRF1 phosphorylation has been implicated in neurological disorders and tumor progression, making this antibody a valuable tool for both basic and translational studies.
×
