Rabbit Polyclonal PKA-R2β(Phospho-Ser114) Antibody

The PKA-R2β (Phospho-Ser114) antibody is a specialized tool used to detect the phosphorylation of the regulatory subunit RIIβ of protein kinase A (PKA) at serine residue 114. PKA, a key enzyme in cAMP-mediated signaling, consists of two regulatory (R) and two catalytic (C) subunits. The RIIβ subunit plays a critical role in anchoring PKA to specific subcellular compartments, thereby localizing its activity. Phosphorylation at Ser114 in RIIβ is a post-translational modification implicated in modulating PKA function, potentially influencing its interaction with A-kinase anchoring proteins (AKAPs) or altering enzymatic activity in response to cellular signals. This antibody is particularly valuable in studies investigating cAMP/PKA signaling dynamics, metabolic regulation, and diseases such as obesity, diabetes, or cancer, where PKA dysregulation is observed. Researchers employ it in techniques like Western blotting, immunoprecipitation, or immunofluorescence to assess phosphorylation status under varying physiological or experimental conditions. Its specificity for the phosphorylated Ser114 epitope is typically validated using knockout controls or phosphatase-treated samples. By enabling precise detection of this modification, the antibody aids in unraveling the mechanistic links between PKA regulation, cellular signaling pathways, and disease pathologies.