| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 1/100-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Alcohol dehydrogenase class 4 mu/sigma chain, Alcohol dehydrogenase class IV mu/sigma chain, Gastric alcohol dehydrogenase, Retinol dehydrogenase, ADH7 |
| Entrez GeneID | 131 |
| WB Predicted band size | 41.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ADH7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 318-346 amino acids from the C-terminal region of human ADH7. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于B-RAF (Phospho-Thr599)抗体的3篇代表性文献的简要总结(注：Thr599磷酸化位点可能存在物种差异或文献命名差异，以下内容基于模拟学术文献示例)：
---
1. **文献名称**: *"Phosphorylation of B-RAF at Thr599 is critical for ERK activation in melanoma cells"*
**作者**: Davies H, et al.
**摘要**: 本研究揭示了B-RAF蛋白在Thr599位点的磷酸化对其激酶活性的调控作用。通过特异性抗体检测发现，该位点的磷酸化在黑色素瘤细胞中显著上调，并依赖上游激酶(如C-RAF)的激活，进而驱动下游ERK信号通路，促进肿瘤生长。
2. **文献名称**: *"Development of a phospho-specific antibody for B-RAF Thr599 and its application in colorectal cancer"*
**作者**: Wan PT, et al.
**摘要**: 研究团队开发了一种针对B-RAF Thr599磷酸化位点的单克隆抗体，验证了其特异性及在结直肠癌组织中的检测效果。结果显示，Thr599磷酸化水平与KRAS突变状态相关，可能作为靶向治疗的潜在生物标志物。
3. **文献名称**: *"Dynamic phosphorylation of B-RAF Thr599 regulates MAPK/ERK signaling in neuronal differentiation"*
**作者**: Yoon S, et al.
**摘要**: 通过神经元分化模型，研究发现B-RAF Thr599的磷酸化呈现动态变化，并受PI3K/AKT通路交叉调控。使用Phospho-Thr599抗体证实，该修饰对神经突触延伸和ERK信号的时间依赖性激活至关重要。
---
**说明**：
- 上述文献为示例性内容，实际研究中B-RAF更常见的磷酸化位点为Thr598/Ser601(人类编号)，可能与用户提到的Thr599存在物种或文献编号差异。
- 建议通过PubMed或抗体供应商官网(如CST、Abcam)查询具体抗体对应的参考文献，或核实磷酸化位点的标准命名。
The B-RAF (Phospho-Thr599) antibody is a specialized tool used to detect the phosphorylated form of the B-RAF protein at threonine residue 599. B-RAF, a serine/threonine kinase within the RAF family, plays a pivotal role in the MAPK/ERK signaling pathway, regulating cell growth, differentiation, and survival. Phosphorylation at Thr599 (or adjacent residues, depending on isoform numbering) is critical for B-RAF activation, as it induces conformational changes that enhance kinase activity and downstream signaling. Dysregulation of B-RAF, particularly through mutations or aberrant phosphorylation, is implicated in numerous cancers, including melanoma, colorectal cancer, and thyroid carcinoma.
This antibody specifically recognizes B-RAF when phosphorylated at Thr599. enabling researchers to study the activation status of B-RAF in cellular or tissue samples. It is widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate pathway activity in cancer models, drug response studies, or mechanistic research. Its utility extends to evaluating therapeutic interventions targeting the MAPK pathway, such as BRAF or MEK inhibitors.
Validation of the antibody typically includes testing in knockout cell lines or phosphopeptide competition assays to ensure specificity. Researchers must consider potential cross-reactivity with related phosphorylated kinases and verify isoform-specificity, as numbering variations exist across B-RAF isoforms. Overall, this antibody serves as a key reagent in dissecting B-RAF's role in health and disease.
×
