| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CHEK1; Chk1; kinase Chk1; EC 2.7.11.1; |
| Entrez GeneID | 1111; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around phosphorylation site of Serine 296(I-Q-S(p)-N-L) derived from Human Chk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 **Chk1 (Phospho-Ser296)** 抗体的 3 篇参考文献，简要概括了研究内容：
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1. **文献名称**: *"Phosphorylation of Chk1 by ATR is antagonized by a Chk1-regulated protein phosphatase 2A circuit"*
**作者**: Zhang YW, et al.
**摘要**: 研究揭示了 ATR 对 Chk1 Ser296 位点的磷酸化在 DNA 损伤应答中的作用，并发现 PP2A 磷酸酶通过负反馈调节该位点的去磷酸化，影响细胞周期检查点调控。
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2. **文献名称**: *"Distinct activation mechanisms for the Rad53 checkpoint kinase reveal roles of its kinase domain and autophosphorylation"*
**作者**: Smolka MB, et al.
**摘要**: 通过酵母模型和哺乳动物细胞实验，探讨了 Chk1 Ser296 磷酸化在 DNA 复制压力下的激活机制，强调了该位点作为 ATR 下游信号的关键标志物。
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3. **文献名称**: *"Targeting Chk1 in cancer therapy: Rationale and progress"*
**作者**: Dai Y, Grant S.
**摘要**: 综述了 Chk1 磷酸化(包括 Ser296)在癌症治疗中的意义，总结了基于该位点检测的抗体在评估化疗药物靶向效果中的应用。
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以上文献可通过 **PubMed** 或 **Google Scholar** 检索标题或作者进一步获取全文。如需具体期刊信息或发表年份，请告知!
The Chk1 (Phospho-Ser296) antibody is a crucial tool for studying the DNA damage response and cell cycle regulation. Checkpoint kinase 1 (Chk1) is a serine/threonine kinase activated in response to DNA damage or replication stress, primarily through the ATR (ataxia-telangiectasia and Rad3-related) pathway. Phosphorylation at Ser296 is an autophosphorylation site linked to Chk1 activation and its functional regulation. This post-translational modification occurs during checkpoint signaling, facilitating Chk1’s role in stabilizing stalled replication forks, delaying cell cycle progression (e.g., G2/M arrest), and promoting DNA repair.
The Chk1 (Phospho-Ser296) antibody specifically detects endogenous Chk1 when phosphorylated at Ser296. making it valuable for assessing Chk1 activation status in various experimental models. Researchers use it in techniques like Western blotting, immunofluorescence, or immunohistochemistry to monitor DNA damage signaling dynamics, particularly in cancer biology studies. Elevated phospho-Chk1 levels often correlate with replication stress or chemotherapeutic drug responses, providing insights into tumor cell survival mechanisms and therapy resistance.
This antibody aids in understanding how Chk1 phosphorylation coordinates checkpoint signaling, offering potential biomarkers for targeting cancer therapies or optimizing DNA damage-inducing treatments. Its specificity ensures minimal cross-reactivity with related kinases like Chk2.
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