Rabbit Polyclonal Bax(Phospho-Thr167) Antibody

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     Immunofluorescence staining of methanol-fixed A549 cells using Bax (Phospho-Thr167) Antibody P40392.    

The Bax (Phospho-Thr167) antibody is a specialized tool used to detect the phosphorylated form of the Bax protein at threonine residue 167. Bax, a pro-apoptotic member of the BCL-2 family, plays a critical role in mitochondrial-mediated apoptosis. Under normal conditions, Bax resides in the cytosol but undergoes conformational changes and translocates to the mitochondrial membrane upon apoptotic stimuli, triggering cytochrome c release and caspase activation. Phosphorylation at Thr167 is a post-translational modification implicated in regulating Bax activity, potentially influencing its oligomerization, subcellular localization, or interaction with other BCL-2 family proteins. Studies suggest this phosphorylation event may enhance Bax’s pro-apoptotic function, though its precise mechanistic role remains under investigation.

The Phospho-Thr167-specific antibody enables researchers to study Bax activation dynamics in response to cellular stress, DNA damage, or chemotherapeutic agents. It is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to assess Bax phosphorylation status in cell lines, tissues, or experimental models. This antibody is particularly valuable in cancer research, neurodegenerative disease studies, and investigations of apoptosis dysregulation. Its specificity for the phosphorylated epitope requires validation via controls (e.g., phosphatase treatment or non-phosphorylated peptide competition) to ensure accurate detection. Understanding Bax phosphorylation at Thr167 contributes to insights into cell fate decisions and therapeutic strategies targeting apoptotic pathways.