| WB | 咨询技术 | Human,Mouse,Rat Monkey |
| IF | 咨询技术 | Human,Mouse,Rat Monkey |
| IHC | 咨询技术 | Human,Mouse,Rat Monkey |
| ICC | 1/100-1/200 | Human,Mouse,Rat Monkey |
| FCM | 咨询技术 | Human,Mouse,Rat Monkey |
| Elisa | 咨询技术 | Human,Mouse,Rat Monkey |
| Aliases | CHEK2; CHK2; Cds1; Chk2; RAD53 |
| Entrez GeneID | 11200; |
| WB Predicted band size | 60kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat Monkey |
| Immunogen | Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Chk2 (Phospho-Thr383)抗体的3篇参考文献，包含文献名称、作者及摘要内容概括：
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1. **文献名称**: **"Phosphorylation of Chk2 by ATM-DNA damage response pathway"**
**作者**: Matsuoka, S. et al.
**摘要**: 本研究揭示了在DNA损伤后，ATM激酶通过磷酸化Chk2的Thr383位点激活其功能。作者利用Phospho-Thr383特异性抗体，通过Western blot和免疫荧光验证了该位点的磷酸化对Chk2激酶活性及细胞周期检查点调控的关键作用。
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2. **文献名称**: **"Role of Chk2 phosphorylation in p53-dependent apoptosis"**
**作者**: Hirao, A. et al.
**摘要**: 文章通过使用Phospho-Thr383抗体，证明电离辐射诱导的Chk2 Thr383磷酸化是p53依赖的凋亡信号通路中的必要步骤。实验表明，该位点的突变显著抑制了DNA损伤后Chk2与p53的结合能力。
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3. **文献名称**: **"Validation of phospho-specific antibodies for Chk2 signaling studies"**
**作者**: Schwarz, J.K. et al.
**摘要**: 本文系统评估了多种Chk2磷酸化抗体(包括Thr383位点)的特异性，通过体外激酶实验和点突变细胞系验证其可靠性。研究强调Phospho-Thr383抗体在检测内源性Chk2激活中的高灵敏度与特异性。
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**备注**：若需获取全文或更多文献，建议通过PubMed或Sci-Hub输入关键词(如“Chk2 Thr383 phosphorylation”)进一步筛选。部分研究可能因抗体商品化命名差异(如pT383 vs. pThr383)需调整检索策略。
The Chk2 (Phospho-Thr383) antibody is a crucial tool for studying the DNA damage response pathway. Checkpoint kinase 2 (Chk2) is a serine/threonine kinase activated in response to DNA double-strand breaks, playing a central role in cell cycle arrest, DNA repair, and apoptosis. Phosphorylation at threonine 383 (Thr383) is a critical post-translational modification required for Chk2 activation. This event occurs downstream of ATM kinase, which phosphorylates Chk2 at Thr68 in response to DNA damage, leading to Chk2 dimerization and autophosphorylation at Thr383/Thr387. The Chk2 (Phospho-Thr383) antibody specifically detects this activated form, making it valuable for monitoring Chk2 activation status in experimental models.
Researchers use this antibody in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate DNA damage signaling in cancer biology, genotoxic stress responses, and therapeutic resistance. It helps assess Chk2's role in tumor suppression, as Chk2 mutations or dysregulation are linked to Li-Fraumeni syndrome and various cancers. The antibody's specificity for the phosphorylated form allows differentiation between active and inactive Chk2. providing insights into temporal activation patterns following DNA-damaging agents like radiation or chemotherapy drugs. Validation typically includes testing in Chk2-deficient cells or using phosphatase treatment to confirm phosphorylation dependency. Its applications extend to studying crosstalk between Chk2 and other DNA repair proteins like p53. BRCA1. and CDC25.
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