关键信息
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基因名
HK1
- 应用
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别名
HK1;Hexokinase-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P19367
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表达区间
13-475aa
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氨基酸序列
ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA
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分子量
55.9kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
HK1, or Hexokinase 1, is a crucial enzyme in the glycolytic pathway, responsible for phosphorylating glucose to produce glucose-6-phosphate, which is a vital step in cellular energy metabolism. The study of HK1 has garnered significant interest due to its role in cancer metabolism, as many tumors exhibit altered glycolytic activity, often referred to as the Warburg effect. This metabolic shift allows cancer cells to thrive in low-oxygen environments and supports rapid proliferation. Furthermore, HK1 has been implicated in a variety of cellular processes, including apoptosis and survival signaling, making it a potential therapeutic target. Researchers have focused on characterizing HK1's structure and function to better understand its regulatory mechanisms, interactions with other proteins, and its potential as a biomarker for disease progression. The exploration of HK1's role in metabolic reprogramming has also prompted investigations into its effects on cellular functions beyond energy metabolism, including its involvement in aging and neurodegenerative diseases. Given the enzyme's implications in diverse pathological conditions, the development of recombinant HK1 for high-throughput screening in drug discovery has become a promising research avenue, aiming to identify small molecules that can modulate its activity. Ultimately, this research not only deepens our understanding of metabolic regulation in health and disease but also opens new avenues for targeted therapies that could improve clinical outcomes for patients with metabolic disorders and various cancers.
