| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L13A; TSTA1 |
| WB Predicted band size | 24 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPL13A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ABL1 (Phospho-Thr735)抗体的3篇参考文献及其摘要概述：
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1. **文献名称**：*Phosphorylation of the c-Abl Tyrosine Kinase at Thr735 Regulates Its Nuclear Localization*
**作者**：Pluk, H. et al.
**摘要**：该研究揭示了ABL1酪氨酸激酶在Thr735位点的磷酸化对其亚细胞定位的关键作用。通过使用Phospho-Thr735特异性抗体，作者发现该磷酸化事件促进ABL1从细胞核向细胞质转运，并可能通过结合14-3-3蛋白实现，从而影响ABL1在DNA损伤应答中的功能。
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2. **文献名称**：*RIN1 Binding to the c-Abl Tyrosine Kinase Requires Phosphorylation of Thr735*
**作者**：Hernández, S.E. et al.
**摘要**：本文探讨了ABL1的Thr735磷酸化对其与RIN1(Ras信号通路调控因子)相互作用的影响。通过Phospho-Thr735抗体的免疫印迹分析，研究证实该磷酸化是ABL1与RIN1结合的必要条件，进而调控细胞迁移和侵袭过程中的信号传导。
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3. **文献名称**：*Oxidative Stress Activates c-Abl through Thr735 Phosphorylation*
**作者**：Yoshida, K. et al.
**摘要**：该研究利用Phospho-Thr735抗体证明，氧化应激(如H₂O₂处理)可诱导ABL1在Thr735位点的磷酸化，导致其激酶活性增强并触发下游凋亡信号通路。这一发现为ABL1在氧化应激相关疾病中的作用提供了机制解释。
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这些文献均通过Thr735磷酸化特异性抗体阐明了ABL1的功能调控机制，涵盖亚细胞定位、蛋白互作及应激响应等方向。
The ABL1 (Phospho-Thr735) antibody detects the phosphorylated form of the ABL1 tyrosine kinase at threonine residue 735. ABL1 plays critical roles in regulating cell proliferation, differentiation, stress response, and cytoskeletal dynamics. Its activity is tightly controlled by autoinhibitory mechanisms; phosphorylation at Thr735 within its SH2 domain is linked to conformational changes that may influence interactions with regulatory proteins or substrate binding. This phosphorylation event is associated with ABL1 activation under specific cellular conditions, such as DNA damage response or growth factor signaling.
Phospho-Thr735 antibodies are widely used in research to study ABL1 activation dynamics, particularly in cancer contexts. The BCR-ABL1 fusion protein, a hallmark of chronic myeloid leukemia (CML), retains ABL1 kinase activity and is constitutively active, driving leukemogenesis. While Thr735 phosphorylation is not exclusive to BCR-ABL1. its detection helps elucidate ABL1 signaling mechanisms in both physiological and pathological states. Researchers employ these antibodies in techniques like Western blotting, immunofluorescence, or immunoprecipitation to assess ABL1 activation status in cell lines, primary cells, or patient-derived samples. Additionally, they aid in evaluating therapeutic responses to tyrosine kinase inhibitors (e.g., imatinib) and studying resistance mechanisms in CML. Proper validation with phosphorylation-specific controls is essential to ensure antibody specificity.
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