| 纯度 | > 85 % SDS-PAGE. |
| 种属 | Human |
| 靶点 | BUD31 |
| Uniprot No | P41223 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-144aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSMPKVKRS RKAPPDGWEL IEPTLDELDQ KMREAETEPH EGKRKVESLW PIFRIHHQKT RYIFDLFYKR KAISRELYEY CIKEGYADKN LIAKWKKQGY ENLCCLRCIQ TRDTNFGTNC ICRVPKSKLE VGRIIECTHC GCRGCSG |
| 预测分子量 | 19 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与BUD31重组蛋白相关的文献摘要概括：
1. **文献名称**：BUD31 Promotes Cell Proliferation and Metastasis via Activating the AKT Pathway in Colorectal Cancer
**作者**：Chen X, et al.
**摘要**：研究发现BUD31在结直肠癌细胞中过表达，通过激活AKT信号通路促进肿瘤细胞增殖和转移，重组BUD31蛋白体外实验证实其调控肿瘤恶性表型。
2. **文献名称**：A role for the spliceosome component BUD31 in maintaining pluripotency in human embryonic stem cells
**作者**：Li H, et al.
**摘要**：利用重组BUD31蛋白功能实验，证明BUD31通过调控剪接体活性维持胚胎干细胞的自我更新能力，其缺失导致多能性相关基因剪接异常。
3. **文献名称**：Structural insights into the interaction of BUD31 with the spliceosome core complex
**作者**：Park JH, et al.
**摘要**：通过重组BUD31蛋白与剪接体复合物的体外结合实验，解析了BUD31在pre-mRNA剪接中稳定剪接体结构的功能位点及分子机制。
注：BUD31相关研究多集中于RNA剪接调控及癌症领域，以上文献均为假设性示例，实际文献需通过PubMed/Google Scholar以关键词"BUD31 recombinant"检索确认。
**Background of BUD31 Recombinant Protein**
BUD31. also known as C9orf80 or SAP30L, is a conserved eukaryotic protein encoded by the *BUD31* gene. Initially identified in yeast for its role in bud-site selection and cell cycle regulation, human BUD31 has emerged as a multifunctional player in critical cellular processes, particularly pre-mRNA splicing. It is a core component of the U4/U6.U5 tri-snRNP complex within the spliceosome, facilitating the assembly and catalytic activation required for intron removal during RNA splicing. This involvement underscores its importance in maintaining RNA processing fidelity and gene expression regulation.
Recombinant BUD31 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to study its molecular interactions, structural features, and functional roles. Purified BUD31 often includes affinity tags (e.g., His-tag) for streamlined isolation. Research highlights its association with cancer progression, where dysregulation correlates with abnormal splicing events, uncontrolled proliferation, and resistance to apoptosis. Overexpression of BUD31 has been observed in malignancies like breast and colorectal cancers, positioning it as a potential therapeutic target or biomarker.
Beyond splicing, BUD31 interacts with proteins involved in DNA repair, stress responses, and epigenetic regulation, suggesting broader functional versatility. Studies utilizing recombinant BUD30 enable mechanistic insights into these pathways, drug discovery efforts, and exploration of its dual roles in homeostasis and disease. Its conserved nature across species further supports its utility in comparative models to unravel evolutionarily conserved splicing mechanisms. Overall, BUD31 recombinant protein serves as a vital tool for dissecting splicing-related pathologies and developing targeted interventions.
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