| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | AIF4; AIP4; NAPP1; |
| Entrez GeneID | 83737; |
| WB Predicted band size | 102kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of tyrosine 420(F-I-Y(p)-G-N) derived from Human ITCH . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于 ITCH (Phospho-Tyr420) 抗体的参考文献示例(注：文献标题和作者为虚构示例，仅供参考格式)：
1. **"Phosphorylation of ITCH at Tyrosine 420 Regulates Its E3 Ubiquitin Ligase Activity in T-Cell Signaling"**
- **作者**: Smith A, et al.
- **摘要**: 本研究揭示了 ITCH 在 Tyr420 位点的磷酸化对其泛素连接酶活性的调控机制，通过磷酸化特异性抗体验证了该修饰在 T 细胞活化中的功能，并证明其与 Lck 激酶的相互作用。
2. **"A Novel Role of ITCH in EGFR Trafficking via Phosphorylation-Dependent Interaction"**
- **作者**: Chen B, et al.
- **摘要**: 文章利用 Phospho-Tyr420 抗体证明 EGFR 信号通路中 ITCH 的磷酸化状态影响其与网格蛋白的相互作用，进而调控受体内吞和降解过程。
3. **"Development and Validation of a Phospho-Specific Antibody for ITCH Tyrosine 420 in Autoimmune Disease Models"**
- **作者**: Lee C, et al.
- **摘要**: 描述了针对 ITCH Tyr420 磷酸化位点的抗体开发流程，并通过免疫印迹和免疫组化验证其在类风湿性关节炎模型中的应用，证明磷酸化 ITCH 与炎症因子释放相关。
4. **"ITCH Phosphorylation at Y420 Modulates Regulatory T Cell Function via Foxp3 Ubiquitination"**
- **作者**: Wang D, et al.
- **摘要**: 研究通过 Phospho-Tyr420 抗体发现 ITCH 的磷酸化状态影响其对 Foxp3 的泛素化修饰，从而调控调节性 T 细胞(Treg)的免疫抑制功能。
**注意**：以上为模拟示例，实际文献需通过 PubMed、Google Scholar 等平台检索关键词(如 "ITCH Phospho-Tyr420 antibody" 或 "ITCH Y420 phosphorylation")获取。建议结合具体研究领域筛选高引论文或权威期刊文章。
The ITCH (Phospho-Tyr420) antibody is designed to detect ITCH protein phosphorylated at tyrosine residue 420. a post-translational modification critical for regulating its enzymatic activity. ITCH, an E3 ubiquitin ligase of the Nedd4 family, plays key roles in ubiquitination-mediated protein degradation, influencing processes like immune response, cell cycle control, and apoptosis. Phosphorylation at Tyr420. often induced by tyrosine kinases such as JAK3 or Src family kinases, enhances ITCH's catalytic activity by relieving autoinhibitory interactions, enabling substrate recognition and polyubiquitination.
This antibody is particularly valuable for studying ITCH activation mechanisms in signaling pathways. Dysregulation of ITCH phosphorylation is linked to autoimmune diseases (e.g., lupus), cancers, and neurological disorders. For example, phosphorylated ITCH promotes T-cell anergy by ubiquitinating TCR signaling components, while its loss correlates with tumor progression via impaired degradation of oncoproteins.
Researchers use the ITCH (Phospho-Tyr420) antibody in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate phosphorylation-dependent interactions or activity in cell lines, tissues, or disease models. Proper controls (e.g., phosphatase treatment) are essential to confirm specificity. Its development has advanced understanding of how post-translational modifications fine-tune ubiquitin ligase function in health and disease.
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