Rabbit Polyclonal EphrinB1/B2/B3(phosphoY324/Y311/Y318) Antibody

The Ephrin B1/B2/B3 (phospho Y324/Y311/Y318) antibody detects site-specific phosphorylation of Ephrin B family transmembrane ligands, which play critical roles in bidirectional cell signaling through interactions with Eph receptor tyrosine kinases. Ephrin B proteins (B1. B2. B3) share a conserved cytoplasmic domain containing tyrosine residues (Y324 in B1. Y311 in B2. Y318 in B3) that undergo phosphorylation to mediate "reverse signaling." These phosphorylated tyrosines serve as docking sites for SH2 domain-containing adaptor proteins, such as Grb4. facilitating downstream pathways like Rho GTPase, MAPK, and cytoskeletal remodeling. This signaling regulates cell-cell communication, migration, and tissue morphogenesis during neurodevelopment, angiogenesis, and tumor progression. Phosphorylation is triggered by Eph receptor engagement or crosstalk with other kinases (e.g., Src). The antibody specifically recognizes phosphorylated forms of all three Ephrin B isoforms, enabling researchers to study their activation dynamics in contexts like neural connectivity, vascular patterning, or cancer metastasis. Applications include Western blotting, immunofluorescence, and investigating therapeutic targets for diseases involving dysregulated Ephrin-Eph signaling. Validation requires controls like dephosphorylation treatments or non-phosphorylated peptide competition to confirm specificity, as phosphorylation is transient and context-dependent.