Rabbit Polyclonal Claudin3(Phospho-Tyr219) Antibody

Claudin-3. a key component of tight junctions, plays a critical role in regulating paracellular permeability and maintaining epithelial/endothelial barrier integrity. Its phosphorylation at Tyr219 (Y219) is a post-translational modification implicated in dynamic junctional remodeling. This phosphorylation event is regulated by tyrosine kinase signaling pathways, including Src family kinases or growth factor receptors, which modulate cell-cell adhesion in response to physiological or pathological stimuli. The Claudin-3 (Phospho-Tyr219) antibody specifically detects this phosphorylated form, enabling researchers to study its functional significance in contexts such as cancer metastasis (e.g., breast or colorectal cancers), inflammatory bowel disease, or infection-induced barrier disruption. Phosphorylation at Y219 has been associated with altered tight junction stability, enhanced epithelial-mesenchymal transition (EMT), and increased paracellular transport. The antibody is widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate signaling crosstalk in epithelial polarization, drug permeability studies, or therapeutic targeting of pathological barrier dysfunction. Validation typically includes knockout controls or phosphatase-treated samples to confirm phosphorylation-specific binding. Its applications extend to evaluating potential therapies aiming to restore barrier function or inhibit cancer progression through claudin-3 modulation.