| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | E12; Tran; TCF3; TFE2; ITF1 |
| Entrez GeneID | 6929; |
| WB Predicted band size | 67kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around phosphorylation site of threonine 355 (P-S-T(p)-P-V) derived from Human E2A . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于E2A (Phospho-Thr355)抗体的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库验证)：
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1. **Title**: "Phosphorylation of E2A at Thr355 Modulates Transcriptional Activity in B Cell Development"
**Authors**: Smith J, Doe R, Lee K.
**Summary**: 本研究利用E2A (Phospho-Thr355)特异性抗体，揭示了Thr355磷酸化通过抑制E2A与DNA结合的能力调控B细胞分化的机制，并发现该修饰受B细胞受体信号通路调控。
2. **Title**: "Characterization of a Phosphospecific Antibody for E2A Thr355 and Its Application in Leukemia Research"
**Authors**: Brown T, Wilson H, Chen X.
**Summary**: 文章详细描述了E2A (Phospho-Thr355)抗体的制备与验证，并应用该抗体在急性淋巴细胞白血病样本中检测到异常磷酸化E2A蛋白，提示其与疾病进展相关。
3. **Title**: "MAPK-Mediated Phosphorylation of E2A at Thr355 Promotes Protein Degradation via the Ubiquitin-Proteasome Pathway"
**Authors**: Zhang Y, Gupta P, Kim S.
**Summary**: 通过免疫印迹和免疫荧光实验(使用Phospho-Thr355抗体)，研究发现MAPK信号通路诱导的Thr355磷酸化促进E2A泛素化及降解，影响淋巴细胞增殖。
4. **Title**: "Dynamic Phosphorylation of E2A Proteins during the Cell Cycle"
**Authors**: Johnson M, White E, García A.
**Summary**: 利用Phospho-Thr355抗体，作者发现E2A在G1/S期转换时发生磷酸化，提示该修饰可能通过调控E2A与ID蛋白的相互作用影响细胞周期进程。
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**建议**：实际文献可通过以下方式查找：
- 在PubMed或Google Scholar中搜索 **"E2A Phospho-Thr355"** 或 **"TCF3 phosphorylation Threonine 355"**;
- 查看抗体供应商(如CST、Abcam)的产品说明书引用文献;
- 结合相关信号通路关键词(如B cell signaling, MAPK, ubiquitination)。
如需具体文献辅助，请提供更多研究背景或访问权限信息。
The E2A (Phospho-Thr355) antibody is designed to detect the transcription factor E2A (also known as TCF3) when phosphorylated at threonine residue 355. E2A, a member of the E-protein family, plays critical roles in B-cell and T-cell development by regulating gene expression through binding to E-box DNA motifs. Its activity is tightly controlled by post-translational modifications, including phosphorylation, which modulates protein stability, interaction partners, and subcellular localization.
Phosphorylation at Thr355 is implicated in regulating E2A's transcriptional activity and turnover. Studies suggest that this modification may influence E2A's ability to dimerize with other transcription factors or recruit co-regulators, thereby affecting lymphocyte differentiation and oncogenic processes. Dysregulation of E2A phosphorylation has been observed in hematologic malignancies, particularly acute lymphoblastic leukemia (ALL), where aberrant E2A fusion proteins or altered signaling pathways may disrupt normal phosphorylation dynamics.
The E2A (Phospho-Thr355) antibody serves as a tool for investigating E2A's phosphorylation status in cellular signaling studies, cancer research, and immune system development. It is commonly used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to explore phosphorylation-dependent mechanisms underlying E2A's biological functions and pathological roles.
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