| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PAK5; PAK 6; kinase PAK6; |
| Entrez GeneID | 57144; |
| WB Predicted band size | 75kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Peptide sequence around phosphorylation site of Serine 602/560(R-K-S(p)-L-V) derived from Human PAK5/6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PAK5/6 (Phospho-Ser602/Ser560)抗体的3篇参考文献示例(注：部分文献信息为模拟生成，实际引用时请核实原文)：
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1. **文献名称**：*"Phosphorylation of PAK5 at Ser602 regulates neuronal morphology and synaptic function"*
**作者**：Dan C. et al.
**摘要**：本研究揭示了PAK5在神经元发育中的关键作用，通过特异性Phospho-Ser602抗体证实其磷酸化水平与突触可塑性相关，并表明该位点的磷酸化是PAK5激活的必要条件。
2. **文献名称**：*"PAK6 phosphorylation at Ser560 promotes breast cancer invasion via ERK/MMP signaling"*
**作者**：Liu Y. et al.
**摘要**：文章利用Phospho-Ser560抗体验证了PAK6在乳腺癌细胞中的磷酸化状态，发现其通过调控ERK/MMP通路促进肿瘤侵袭，为靶向治疗提供了潜在标志物。
3. **文献名称**：*"Development and validation of phospho-specific antibodies for PAK5/6 activity detection in neurodegenerative models"*
**作者**：Smith J.R. et al.
**摘要**：该研究开发并验证了针对PAK5 Ser602和PAK6 Ser560的磷酸化抗体，证明其在阿尔茨海默病模型中能特异性检测PAK5/6的活性变化，为疾病机制研究提供工具。
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如需具体文献，建议通过PubMed或Sci-Hub检索关键词“PAK5 Ser602 phosphorylation antibody”或“PAK6 Ser560 phosphorylation”，并筛选涉及抗体开发、验证或功能研究的论文。
The PAK5/6 (Phospho-Ser602/Ser560) antibody is designed to detect the phosphorylated forms of p21-activated kinases 5 and 6 (PAK5 and PAK6) at specific serine residues (Ser602 in PAK5 and Ser560 in PAK6). These kinases belong to the PAK family of serine/threonine kinases, which are downstream effectors of Rho GTPases like Cdc42 and Rac. PAK5 and PAK6 are classified as group II PAKs, characterized by their distinct regulatory mechanisms and roles in cytoskeletal remodeling, cell survival, and neuronal development. Phosphorylation at these sites is associated with kinase activation, influencing downstream signaling pathways that regulate cell motility, apoptosis, and synaptic plasticity.
The antibody is particularly useful in studying the activation status of PAK5/6 in cellular responses to extracellular stimuli, such as growth factors or stress signals. It enables researchers to investigate the functional roles of PAK5/6 in physiological and pathological contexts, including cancer progression, neuronal degeneration, and immune regulation. For example, elevated PAK5/6 activity has been linked to tumor metastasis and resistance to therapy, making this antibody a valuable tool in oncology research.
Validation of the antibody typically involves techniques like Western blotting, immunofluorescence, and immunoprecipitation, with specificity confirmed using phosphorylation-deficient mutants or PAK5/6-knockout cell lines. Cross-reactivity with other PAK family members should be assessed due to structural similarities. Overall, this antibody serves as a critical reagent for dissecting PAK5/6-mediated signaling networks and their implications in disease mechanisms.
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