关键信息
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基因名
PXN
- 应用
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别名
PXN；Paxillin
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
P49023
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表达区间
1-591aa
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氨基酸序列
MDDLDALLAD LESTTSHISK RPVFLSEETP YSYPTGNHTY QEIAVPPPVP PPPSSEALNG TILDPLDQWQ PSSSRFIHQQ PQSSSPVYGS SAKTSSVSNP QDSVGSPCSR VGEEEHVYSF PNKQKSAEPS PTVMSTSLGS NLSELDRLLL ELNAVQHNPP GFPADEANSS PPLPGALSPL YGVPETNSPL GGKAGPLTKE KPKRNGGRGL EDVRPSVESL LDELESSVPS PVPAITVNQG EMSSPQRVTS TQQQTRISAS SATRELDELM ASLSDFKIQG LEQRADGERC WAAGWPRDGG RSSPGGQDEG GFMAQGKTGS SSPPGGPPKP GSQLDSMLGS LQSDLNKLGV ATVAKGVCGA CKKPIAGQVV TAMGKTWHPE HFVCTHCQEE IGSRNFFERD GQPYCEKDYH NLFSPRCYYC NGPILDKVVT ALDRTWHPEH FFCAQCGAFF GPEGFHEKDG KAYCRKDYFD MFAPKCGGCA RAILENYISA LNTLWHPECF VCRECFTPFV NGSFFEHDGQ PYCEVHYHER RGSLCSGCQK PITGRCITAM AKKFHPEHFV CAFCLKQLNK GTFKEQNDKP YCQNCFLKLF C
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分子量
64.5 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
PXN (paxillin) is a focal adhesion-adapter protein that plays a crucial role in integrin signaling, cell adhesion, and migration processes. Its significance in cellular responses to the extracellular environment has made it a focal point of research, especially in the context of cancer metastasis, wound healing, and development. Studies have shown that PXN is overexpressed in various cancers, contributing to tumor progression and metastasis by modulating signaling pathways that govern cell motility and survival. Furthermore, PXN interacts with numerous proteins, including tyrosine kinases and actin cytoskeletal components, underscoring its importance in cellular architecture and function. The exploration of PXN's structural characteristics through recombinant protein studies has been instrumental in understanding its interaction networks and regulatory mechanisms. By generating and characterizing PXN recombinant proteins, researchers aim to elucidate the molecular underpinnings of its role in pathological conditions, opening avenues for therapeutic interventions targeting its functional pathways. As PXN continues to receive attention in cell biology and cancer research, its comprehensive investigation promises to yield insights into potential biomarkers and treatment strategies, highlighting its relevance in advancing our understanding of complex cellular behaviors.
